PMID- 9245445
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20171116
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 250
IP  - 2
DP  - 1997 Aug 1
TI  - Separation of oligo/polymers of 5-N-acetylneuraminic acid, 5-N-glycolylneuraminic 
      acid, and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid by high-performance 
      anion-exchange chromatography with pulsed amperometric detector.
PG  - 245-51
AB  - A sensitive and efficient method to analyze oligo/ poly-sialic acids containing 
      alpha2-8-linked 5-N-acetylneuraminic acid (Neu5Ac), 5-N-glycolylneuraminic acid 
      (Neu5Gc), and deaminated neuraminic acid (KDN) using high-performance 
      anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD-2) 
      has been developed. Using a CarboPac PA-100 column and sodium nitrate as the 
      pushing agent, polymers in colominic acid with degree of polymerization (DP) up 
      to 80 were separated in 68 min. A similar DP-based resolution was also obtained 
      on a CarboPac PA-1 column. The elution ladders of the Neu5Ac, Neu5Gc, and KDN 
      series were sufficiently different to be used as diagnostic indices. This 
      technique was applied to identification of the sialic acid components in a 
      polysialoglycoprotein (PSGP) sample as well as monitoring the 
      oligo/poly-KDN-containing fractions during the purification of KDN-containing 
      glycoprotein (KDN-gp). The maximum DPs of oligo-Neu5Gc and oligo-KDN that can be 
      detected in PSGP and KDN-gp hydrolysates were 11 and 8, respectively. The high 
      sensitivity of this method was demonstrated by the quantification of Neu5Ac 
      oligomers. Distributions of the monomer and oligo/polymers in the acid and 
      enzymatic hydrolysates of colominic acid and PSGP under different conditions were 
      also studied.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
FAU - Inoue, Y
AU  - Inoue Y
FAU - Inoue, S
AU  - Inoue S
FAU - Lee, Y C
AU  - Lee YC
LA  - eng
GR  - DK09970/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Anion Exchange Resins)
RN  - 0 (Neuraminic Acids)
RN  - 0 (Sialic Acids)
RN  - 0 (Sugar Acids)
RN  - 1113-83-3 (N-glycolylneuraminic acid)
RN  - 9672K4U8IW (3-deoxyglycero-galacto-nonulosonic acid)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
EIN - Anal Biochem 1997 Oct 15;252(2):326
MH  - Anion Exchange Resins
MH  - Chromatography
MH  - Hydrolysis
MH  - N-Acetylneuraminic Acid/chemistry/*isolation & purification
MH  - Neuraminic Acids/chemistry/*isolation & purification
MH  - Sialic Acids/chemistry/isolation & purification
MH  - Sugar Acids/chemistry/*isolation & purification
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0003-2697(97)92225-9 [pii]
AID - 10.1006/abio.1997.2225 [doi]
PST - ppublish
SO  - Anal Biochem. 1997 Aug 1;250(2):245-51. doi: 10.1006/abio.1997.2225.