PMID- 9246408
OWN - NLM
STAT- MEDLINE
DCOM- 19970926
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 3
DP  - 1997 May
TI  - Regional differences in the compaction of chromatin in human G0/G1 interphase 
      nuclei.
PG  - 157-66
AB  - The large-scale structure of chromatin corresponding to G- and R-bands in human 
      G0/G1 interphase nuclei was compared. Fluorescence in situ hybridization (FISH) 
      was used to measure the interphase distance between 42 pairs of probes separated 
      by 0.1-1.5 Mbp. The probe pairs were derived from 21q22.2 and Xp21.3, G-band 
      positive regions, and from 4p16.3, 6p21.3, and Xq28, R-band positive regions. 
      Distributions of measured interphase distances in all regions approximated a 
      Rayleigh distribution, suggesting that the chromatin follows a random-walk path 
      over this range. A linear correlation of mean-square interphase distance and 
      genomic separation, also indicative of random-walk folding, was observed in all 
      regions. The slope of the correlation observed using probes from G-band regions 
      was systematically lower than that from R-band regions. The difference in the 
      slope between Xp21.3 and Xq28 was particularly striking and was observed in 
      normal fibroblast cells, fixed alternatively with methanol and acetic acid or 
      paraformaldehyde, and HeLa cells. These results demonstrate regional differences 
      in large-scale chromosome structure during interphase, with the more openly 
      configured chromatin corresponding to R-bands.
FAU - Yokota, H
AU  - Yokota H
AD  - Epoch Pharmaceuticals, Bothell, WA, USA.
FAU - Singer, M J
AU  - Singer MJ
FAU - van den Engh, G J
AU  - van den Engh GJ
FAU - Trask, B J
AU  - Trask BJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Chromatin)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromatin/*ultrastructure
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Chromosomes, Human/*ultrastructure
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Chromosomes, Human, Pair 4/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - DNA Probes
MH  - Female
MH  - G1 Phase/genetics
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Male
MH  - Resting Phase, Cell Cycle/genetics
MH  - Tissue Fixation
MH  - X Chromosome/genetics
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1023/a:1018438729203 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 May;5(3):157-66. doi: 10.1023/a:1018438729203.