PMID- 9246411 OWN - NLM STAT- MEDLINE DCOM- 19970926 LR - 20191102 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 5 IP - 3 DP - 1997 May TI - Conserved Y-chromosomal location of TSPY in Bovidae. PG - 182-5 AB - We determined the chromosomal location of TSPY, the testis-specific protein, Y-encoded, by fluorescence in situ hybridization (FISH) to chromosome spreads of cattle, goat and sheep. Using a cloned polymerase chain reaction (PCR) product of one bovine TSPY family member, we were able to show a conserved Y chromosomal localization for TSPY in all three species. In contrast to a limited regional distribution of TSPY FISH signals on the chromosome of man, other primates, great apes, goat and sheep, in cattle TSPY-related sequences appear to be spread over most of the Y chromosome. The painting effect observed in this species reflects the higher complexity of the bovine TSPY gene family, being composed not only of a tandemly repeated cluster, but harbouring a large number of different family members dispersed all over the Y chromosome. FAU - Vogel, T AU - Vogel T AD - Institut fur Humangenetik, Medizinische Hochschule Hannover, Germany. FAU - Borgmann, S AU - Borgmann S FAU - Dechend, F AU - Dechend F FAU - Hecht, W AU - Hecht W FAU - Schmidtke, J AU - Schmidtke J LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 RN - 0 (DNA-Binding Proteins) RN - 0 (Nuclear Proteins) RN - 0 (Sex-Determining Region Y Protein) RN - 0 (Transcription Factors) SB - IM MH - Animals MH - Cattle/*genetics MH - Chromosome Mapping MH - DNA-Binding Proteins/*genetics MH - Goats/*genetics MH - In Situ Hybridization, Fluorescence MH - Male MH - *Nuclear Proteins MH - Sex-Determining Region Y Protein MH - Sheep/*genetics MH - *Transcription Factors MH - Y Chromosome/*genetics EDAT- 1997/05/01 00:00 MHDA- 1997/05/01 00:01 CRDT- 1997/05/01 00:00 PHST- 1997/05/01 00:00 [pubmed] PHST- 1997/05/01 00:01 [medline] PHST- 1997/05/01 00:00 [entrez] AID - 10.1023/a:1018494914182 [doi] PST - ppublish SO - Chromosome Res. 1997 May;5(3):182-5. doi: 10.1023/a:1018494914182.