PMID- 9247333
OWN - NLM
STAT- MEDLINE
DCOM- 19970826
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 124
IP  - 15
DP  - 1997 Aug
TI  - Hepatocyte growth factor (HGF/SF) is a muscle-derived survival factor for a 
      subpopulation of embryonic motoneurons.
PG  - 2903-13
AB  - Muscle-derived factors are known to be important for the survival of developing 
      spinal motoneurons, but the molecules involved have not been characterized. 
      Hepatocyte growth factor/scatter factor (HGF/SF) plays an important role in 
      muscle development and motoneuron axon outgrowth. We show that HGF/SF has potent 
      neurotrophic activity (EC50=2 pM) for a subpopulation (40%) of purified embryonic 
      rat motoneurons. Moreover, HGF/SF is an essential component of muscle-derived 
      support for motoneurons, since blocking antibodies to HGF/SF specifically 
      inhibited 65% of the trophic activity of media conditioned by C2/C7 skeletal 
      myotubes, but did not inhibit the trophic activity secreted by Schwann cell 
      lines. High levels of expression of the HGF/SF receptor c-Met in the spinal cord 
      are restricted to subsets of motoneurons, mainly in limb-innervating segments. 
      Consistent with this distribution, cultured motoneurons from limb-innervating 
      brachial and lumbar segments showed a more potent response to HGF/SF than did 
      thoracic motoneurons. By the end of the period of motoneuron cell death, levels 
      of c-Met mRNA in motoneurons were markedly reduced, suggesting that the effects 
      of HGF/SF may be limited to the period of motoneuron cell death. HGF/SF may play 
      an important role during motoneuron development as a muscle-derived survival 
      factor for a subpopulation of limb-innervating motoneurons.
FAU - Yamamoto, Y
AU  - Yamamoto Y
AD  - INSERM U382, Developmental Biology Institute of Marseille (CNRS-INSERM-Universite 
      de la Mediterranee), Campus de Luminy, France.
FAU - Livet, J
AU  - Livet J
FAU - Pollock, R A
AU  - Pollock RA
FAU - Garces, A
AU  - Garces A
FAU - Arce, V
AU  - Arce V
FAU - deLapeyriere, O
AU  - deLapeyriere O
FAU - Henderson, C E
AU  - Henderson CE
LA  - eng
SI  - GENBANK/D10213
SI  - GENBANK/M17701
SI  - GENBANK/Y00671
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Extremities
MH  - Hepatocyte Growth Factor/metabolism/*pharmacology
MH  - Molecular Sequence Data
MH  - Motor Neurons/cytology/*physiology
MH  - Muscle Fibers, Skeletal/metabolism
MH  - Muscle, Skeletal/*embryology/metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptor Protein-Tyrosine Kinases/genetics
MH  - Spinal Nerves/embryology
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1242/dev.124.15.2903 [doi]
PST - ppublish
SO  - Development. 1997 Aug;124(15):2903-13. doi: 10.1242/dev.124.15.2903.