PMID- 9247614
OWN - NLM
STAT- MEDLINE
DCOM- 19970821
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 377
IP  - 2
DP  - 1997 Jul 3
TI  - Accumulation of genetic alterations in a human hepatoma cell line transfected 
      with hepatitis B virus.
PG  - 187-98
AB  - Chromosome and molecular analyses of the hepatitis B virus (HBV)-transfected 
      HepG2T14.1 variant of the HepG2 cell line was conducted. In HepG2T14.1 cells 
      several genetic alterations such as de novo aberrations of chromosomes 9, 14, 15, 
      and 20 were identified that are not present in the parental HepG2 cell line. 
      Furthermore, HepG2T14.1 cells showed loss of heterozygosity (LOH) in the q region 
      of chromosome 14. The single HBV integration site in HepG2T14.1 cells mapped to 
      the 2q35-36 region of one copy of chromosome 2 by fluorescence in situ 
      hybridization (FISH). No genetic changes were identified at or near the HBV 
      integration site at the level of these analyses. In addition, growth rates in 
      vivo and in vitro were dramatically accelerated in HepG2T14.1 cells. These 
      results document that a HBV-transfected hepatoma cell line has de novo genetic 
      mutations at several sites of the host genome, one HBV integration site in an 
      non-rearranged chromosome and an altered phenotype. These findings support our 
      hypothesis that HBV might play a role in cellular transformation by interfering 
      with cellular processes responsible for the stability of the genome.
FAU - Livezey, K W
AU  - Livezey KW
AD  - Hahnemann School of Medicine, Department of Pathology and Laboratory Medicine, 
      Philadelphia, PA 19129, USA.
FAU - Simon, D
AU  - Simon D
LA  - eng
GR  - CA58525/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Transposable Elements)
SB  - IM
MH  - Animals
MH  - Carcinogenicity Tests
MH  - Carcinoma, Hepatocellular/*genetics/*virology
MH  - Cell Division/genetics
MH  - Cell Line
MH  - Cell Line, Transformed
MH  - Chromosome Aberrations
MH  - Chromosome Mapping
MH  - DNA Transposable Elements
MH  - Hepatitis B/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mice
MH  - Mice, Nude
MH  - Microsatellite Repeats
MH  - Transfection
EDAT- 1997/07/03 00:00
MHDA- 1997/07/03 00:01
CRDT- 1997/07/03 00:00
PHST- 1997/07/03 00:00 [pubmed]
PHST- 1997/07/03 00:01 [medline]
PHST- 1997/07/03 00:00 [entrez]
AID - S0027-5107(97)00068-7 [pii]
AID - 10.1016/s0027-5107(97)00068-7 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Jul 3;377(2):187-98. doi: 10.1016/s0027-5107(97)00068-7.