PMID- 9249065
OWN - NLM
STAT- MEDLINE
DCOM- 19970826
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 193
IP  - 1
DP  - 1997 Jul 1
TI  - Asymmetrical progression of replication forks just after initiation on Mycoplasma 
      capricolum chromosome revealed by two-dimensional gel electrophoresis.
PG  - 39-47
AB  - Previously, we mapped the replication initiation site of the Mycoplasma 
      capricolum chromosome into a region containing the dnaA gene [M. Miyata et al., 
      1993a. Nucleic Acids Res. 21, 4816-4823]. In this study, various regions 
      including this functional domain were analyzed by two complementary 
      two-dimensional (2D) gel electrophoretic methods. Sizes of nascent strands in a 
      10.7-kb and a 5.6-kb region were examined by a neutral/alkaline (N/A) method. The 
      shortest nascent strand was detected in an 875-bp region composed of the 3' end 
      of the dnaA gene and its downstream non-coding sequence. The shortest nascent 
      strand detected became longer in an asymmetrical manner as position of the probe 
      became further from the putative initiation site in both directions. The 
      intermediate forms of eight regions restricted at different sites were examined 
      by a neutral/neutral (N/N) method. Bubble arcs were observed in four regions 
      including the 875-bp region. The region containing the 875-bp region at about its 
      center showed an asymmetrical arc, although that containing the 875-bp region at 
      its end showed a symmetrical arc. These results show that the replication forks 
      develop in the 875-bp region and proceed bidirectionally in an asymmetrical 
      manner around the initiation site. The results of N/A analysis of the 5.6-kb 
      region showed a shift of intensity in the nascent strand signal, which suggests 
      an upshift of fork progression velocity.
FAU - Miyata, M
AU  - Miyata M
AD  - Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 
      Japan. miyata@sci.osaka-cu.ac.jp
FAU - Fukumura, T
AU  - Fukumura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DnaA protein, Bacteria)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Bacterial Proteins/metabolism
MH  - DNA Probes
MH  - *DNA Replication
MH  - DNA, Bacterial/*genetics
MH  - DNA-Binding Proteins/metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Mycoplasma/*genetics
MH  - *Nucleic Acid Conformation
MH  - Sepharose
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - S0378-1119(97)00075-9 [pii]
AID - 10.1016/s0378-1119(97)00075-9 [doi]
PST - ppublish
SO  - Gene. 1997 Jul 1;193(1):39-47. doi: 10.1016/s0378-1119(97)00075-9.