PMID- 9250805
OWN - NLM
STAT- MEDLINE
DCOM- 19970926
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 26
IP  - 1-2
DP  - 1997 Jun
TI  - Sequential interphase FISH analysis of m-BCR/ABL chimeric gene-positive cells in 
      Ph-positive acute myeloid leukemia.
PG  - 185-91
AB  - We report a case of Philadelphia (Ph)-positive AML in which interphase 
      fluorescence in situ hybridization (FISH) analysis was performed from diagnosis 
      throughout the course of therapy using major (M-) breakpoint cluster region 
      (BCR)/minor (m-) BCR and ABL cosmid probes. We also investigated the existence of 
      the M-BCR or m-BCR at the RNA or DNA level by the reverse transcriptase 
      polymerase chain reaction and Southern blot analysis, respectively. Complete 
      remission with a normal karyotype was achieved after several regimens of 
      chemotherapy and peripheral blood stem cell transplantation (PBSCT), but relapse 
      occurred and his cells became 100% Ph-positive. We detected the m-BCR/ABL fusion 
      gene by interphase FISH analysis using an m-BCR/ABL translocation probe, and 
      found that FISH analysis was useful for classifying the BCR, identifying minimal 
      residual disease, and for predicting imminent relapse after chemotherapy and 
      PBSCT.
FAU - Onishi, R
AU  - Onishi R
AD  - Department of Immunology and Hematology, Kitano Hospital, Tazuke-Kofukai Medical 
      Research Institute, Japan.
FAU - Tanaka, K
AU  - Tanaka K
FAU - Shimazaki, C
AU  - Shimazaki C
FAU - Yamagata, N
AU  - Yamagata N
FAU - Konaka, Y
AU  - Konaka Y
FAU - Namiuchi, S
AU  - Namiuchi S
FAU - Kamada, N
AU  - Kamada N
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Antineoplastic Agents)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Acute Disease
MH  - Antineoplastic Agents/therapeutic use
MH  - Chimera
MH  - Combined Modality Therapy
MH  - DNA, Neoplasm/analysis
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/*genetics/therapy
MH  - Male
MH  - Middle Aged
MH  - Neoplasm, Residual/genetics
MH  - Predictive Value of Tests
MH  - RNA, Neoplasm/analysis
MH  - Recurrence
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.3109/10428199709109175 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 1997 Jun;26(1-2):185-91. doi: 10.3109/10428199709109175.