PMID- 9251243
OWN - NLM
STAT- MEDLINE
DCOM- 19970929
LR  - 20190920
IS  - 1357-2725 (Print)
IS  - 1357-2725 (Linking)
VI  - 29
IP  - 5
DP  - 1997 May
TI  - The gene for the human interleukin-11 receptor alpha chain locus is highly 
      homologous to the murine gene and contains alternatively spliced first exons.
PG  - 753-66
AB  - The gene for the murine interleukin-11 receptor alpha chain (mIL-11R alpha) 
      contains two loci (1 and 2), of which locus 2 is restricted to only some mouse 
      strains. Two alternatively spliced exons (1a and 1b) encode the 5' untranslated 
      region (5'UTR) of the murine locus 1. We have characterized the gene for the 
      human interleukin-11 receptor alpha chain locus (hIL-11R alpha), examined its 
      expression by Northern analysis and determined its chromosomal location by 
      fluorescence in situ hybridization. The presence of exon(s) encoding the 5'UTR 
      and mapping of transcription initiation sites was determined by 
      reverse-transcriptase polymerase chain reaction and 5' rapid amplification of 
      cDNA ends (5'RACE) techniques. The human locus spanned 10 kilobasepairs (kb) and 
      consisted of 14 exons. Two alternatively spliced first exons (1a and 1b) encoding 
      the 5'UTR were identified and shared 76 and 73% nucleotide identity with murine 
      exons 1a and 1b. Multiple transcription start sites were demonstrated for human 
      exon 1a. The promoter regions of both human exons 1a and 1b did not display a 
      canonical TATA box. A predominant 1.8 kb transcript for the hIL-11R alpha was 
      present in heart, brain, skeletal muscle, lymph nodes, thymus, appendix, pancreas 
      and foetal liver. The hIL-11R alpha gene was localized to chromosome 9p13. In 
      summary, the hIL-11R alpha gene was highly related to locus 1 of the murine gene 
      and there was no evidence of a second hIL-11R alpha locus.
FAU - Nandurkar, H H
AU  - Nandurkar HH
AD  - Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
FAU - Robb, L
AU  - Robb L
FAU - Nicholl, J K
AU  - Nicholl JK
FAU - Hilton, D J
AU  - Hilton DJ
FAU - Sutherland, G R
AU  - Sutherland GR
FAU - Begley, C G
AU  - Begley CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (IL11RA protein, human)
RN  - 0 (Il11ra1 protein, mouse)
RN  - 0 (Il11ra2 protein, mouse)
RN  - 0 (Interleukin-11 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-11)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 9
MH  - DNA
MH  - *Exons
MH  - Humans
MH  - Interleukin-11 Receptor alpha Subunit
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Receptors, Interleukin/*genetics
MH  - Receptors, Interleukin-11
MH  - Restriction Mapping
MH  - Transcription, Genetic
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S1357-2725(97)00011-3 [pii]
AID - 10.1016/s1357-2725(97)00011-3 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 1997 May;29(5):753-66. doi: 
      10.1016/s1357-2725(97)00011-3.