PMID- 9256964
OWN - NLM
STAT- MEDLINE
DCOM- 19970929
LR  - 20061115
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 58
IP  - 8
DP  - 1997 Aug
TI  - Airborne transmission of Actinobacillus pleuropneumoniae and porcine reproductive 
      and respiratory syndrome virus in nursery pigs.
PG  - 828-32
AB  - OBJECTIVE: To document airborne transmission of Actinobacillus pleuropneumoniae 
      and porcine reproductive and respiratory syndrome virus (PRRSV) infection in 
      nursery pigs. ANIMALS: 32 two-week-old pigs obtained from 3 farms, but with 
      similar Landrace X Yorkshire genetics for trial 1 of each experiment; 16 pigs for 
      trial 2 of the A pleuropneumoniae experiment; and 14 pigs for trial 2 of the 
      PRRSV experiment. PROCEDURE: In experiment 1, pigs were inoculated with A 
      pleuropneumoniae serotype 1 (6/8) or were left as contacts (2/8). At the 
      beginning of trial 1, pigs were seronegative to A pleuropneumoniae serotypes 1 
      and 5 on the basis of results of an ELISA, but had positive results on the A 
      pleuropneumoniae hemolysin I (Apx1)-neutralization test. Pigs in trial 2 had 
      negative results on both tests. Pigs of trial 1 of experiment 2 were inoculated 
      with a PRRSV virulent field isolate (MN-1b); pigs of trial 2 were inoculated with 
      the virus reference strain VR-2332. Aerosol-exposed pigs were placed on the other 
      side of the air duct and kept there for 2 to 7 weeks depending on evidence of 
      airborne transmission. RESULTS: In trial 1 of experiment 1, evidence of airborne 
      transmission was not found. In trial 2, most airborne-exposed pigs died as a 
      result of A pleuropneumoniae infection 12 days after initiation of the 
      experiment. In trial 1 of experiment 2, all inoculated pigs (8/8) seroconverted, 
      but only 2 of 8 contact-exposed pigs seroconverted. Aerosol-exposed pigs did not 
      seroconvert nor was virus isolated. In trial 2, all inoculated and 
      contact-exposed pigs seroconverted. All aerosol-exposed pigs seroconverted after 
      21 days, and virus was isolated at 16 days. CONCLUSIONS: A pleuropneumoniae was 
      transmitted by air at a distance of 1 m when pigs were fully susceptible to the 
      organism. Transmission of PRRSV appeared to be strain dependent; when reference 
      strain VR-2332 was used, airborne transmission of PRRSV was documented.
FAU - Torremorell, M
AU  - Torremorell M
AD  - Department of Clinical and Population Sciences, College of Veterinary Medicine, 
      University of Minnesota, St Paul 55108, USA.
FAU - Pijoan, C
AU  - Pijoan C
FAU - Janni, K
AU  - Janni K
FAU - Walker, R
AU  - Walker R
FAU - Joo, H S
AU  - Joo HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
SB  - IM
MH  - Actinobacillus Infections/transmission/*veterinary
MH  - *Actinobacillus pleuropneumoniae/classification
MH  - Air Microbiology
MH  - Animals
MH  - *Housing, Animal
MH  - *Microclimate
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Serotyping
MH  - Swine
MH  - *Swine Diseases
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
PST - ppublish
SO  - Am J Vet Res. 1997 Aug;58(8):828-32.