PMID- 92576
OWN - NLM
STAT- MEDLINE
DCOM- 19800228
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 32
IP  - 3
DP  - 1979 Dec
TI  - Establishment of a C3Hf mammary tumor cell line expressing endogenous mouse 
      mammary tumor virus: antigenic and genetic relationships of this virus with 
      highly oncogenic mouse mammary tumor viruses.
PG  - 852-9
AB  - A single-cell clone of C3Hf mammary tumor cells (clone 14) was developed into a 
      continuous cell line expressing high levels of endogenous mouse mammary tumor 
      virus (MMTV) with less than 0.1% murine leukemia virus expression. Comparison of 
      the C3Hf MMTV protein profile on sodium dodecyl sulfatepolyacrylamide gel 
      electrophoresis with that of C3H MMTV revealed that the protein content of the 
      two viruses was quite similar. However, oligonucleotide fingerprints obtained of 
      MMTV 70S RNA revealed that approximately 20% of the large oligonucleotides 
      examined were unique to each virus. The oligonucleotide fingerprint indicated 
      that although the viruses were similar, they differed in their genetic content. 
      The differences in the two viruses extended to immunological differences in the 
      major envelope glycoprotein, gp52. C3Hf MMTV competed only partially in a 
      homologous radioimmunoassay for gp52 of C3H MMTV, whereas C3H MMTV gave complete 
      competition, indicating that gp52 of C3H MMTV contained type-specific 
      determinants not present on gp52 of C3Hf MMTV. Comparison of C3Hf MMTV with 
      highly oncogenic C3H, GR, and RIII MMTVs in a homologous C3H MMTV gp52 assay gave 
      two patterns of reactivity: complete competition by GR and C3H MMTV and 
      incomplete competition by C3Hf and RIII MMTV. Absorption of anti-C3H MMTV serum 
      by either C3Hf MMTV or RIII MMTV removed all antibodies against both viruses but 
      not against GR and C3H MMTVs. These results indicate that C3H and GR MMTVs are 
      more closely related to each other than to RIII and C3Hf MMTVs.
FAU - Arthur, L O
AU  - Arthur LO
FAU - Lovinger, G G
AU  - Lovinger GG
FAU - Schochetman, G
AU  - Schochetman G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - *Cell Line
MH  - Epitopes
MH  - Female
MH  - Mammary Neoplasms, Experimental/*microbiology
MH  - Mammary Tumor Virus, Mouse/analysis/*growth & development/immunology
MH  - Mice
MH  - *Mice, Inbred C3H
MH  - RNA, Viral/analysis
MH  - Viral Proteins/analysis
MH  - *Virus Cultivation
PMC - PMC525934
EDAT- 1979/12/01 00:00
MHDA- 1979/12/01 00:01
PMCR- 1979/12/01
CRDT- 1979/12/01 00:00
PHST- 1979/12/01 00:00 [pubmed]
PHST- 1979/12/01 00:01 [medline]
PHST- 1979/12/01 00:00 [entrez]
PHST- 1979/12/01 00:00 [pmc-release]
AID - 10.1128/JVI.32.3.852-859.1979 [doi]
PST - ppublish
SO  - J Virol. 1979 Dec;32(3):852-9. doi: 10.1128/JVI.32.3.852-859.1979.