PMID- 9258404
OWN - NLM
STAT- MEDLINE
DCOM- 19971014
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 133
IP  - 1
DP  - 1997 Aug
TI  - Oxidized low density lipoprotein and very low density lipoprotein enhance 
      expression of monocyte chemoattractant protein-1 in rabbit peritoneal exudate 
      macrophages.
PG  - 31-6
AB  - The migration of monocytes into arterial subendothelial space is one of the 
      earliest events in atherogenesis. Monocyte chemoattractant protein 1 (MCP-1) is a 
      potent monocyte chemoattractant. The purpose of this work was to examine whether 
      oxidized low density lipoprotein (OX-LDL) and very low density lipoprotein 
      (OX-VLDL) have any effect on the expression of MCP-1 mRNA and protein in rabbit 
      peritoneal exudate macrophages. The total RNA was extracted from the macrophages 
      after 24 h exposure to LDL, VLDL, OX-LDL, and OX-VLDL, respectively, and the 
      media (LDL-CM, VLDL-CM, OX-LDL-CM and OX-VLDL-CM) conditioned by the macrophages 
      exposed to the above-mentioned lipoproteins were collected. The MCP-1 mRNA 
      expression in macrophages was examined by Northern blot analysis. Meanwhile, 
      MCP-1 protein in the conditioned media was determined by sandwich ELISA. The 
      chemotactic activity of the conditioned media for monocytes was determined by 
      micropore filter assay. The results revealed that the macrophages can express 
      MCP-1, and 24 h exposure to OX-LDL and OX-VLDL induced a 3.2-fold and a 3.4-fold 
      increase in MCP-1 mRNA expression in macrophages and a 2.2-fold and a 2.5-fold 
      increase in the level of MCP-1 protein in the conditioned media, respectively. 
      However, 24 h exposure to LDL and VLDL only induced a slight increase in the 
      expression of MCP-1 mRNA and protein in macrophages. Furthermore, the migration 
      distance of monocyte induced by OX-LDL-CM and OX-VLDL-CM was longer than that 
      induced by LDL-CM and VLDL-CM, as well as by CM. We conclude that the macrophages 
      can express MCP-1, and OX-VLDL and OX-VLDL induce stronger MCP-1 expression. It 
      suggests that macrophages may amplify the recruitment into subendothelial space, 
      and OX-LDL and OX-VLDL may play an important role in the pathogenesis of 
      atherosclerosis through enhancing the MCP-1 expression in macrophages.
FAU - Wang, G P
AU  - Wang GP
AD  - Department of Pathology, Tongji Medical University, Wuhan, People's Republic of 
      China. Wanggp@hotmail.com
FAU - Deng, Z D
AU  - Deng ZD
FAU - Ni, J
AU  - Ni J
FAU - Qu, Z L
AU  - Qu ZL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (oxidized low density lipoprotein)
RN  - 0 (oxidized very low density lipoprotein)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Chemotaxis, Leukocyte
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exudates and Transudates/metabolism
MH  - Lipoproteins, LDL/*pharmacology
MH  - Lipoproteins, VLDL/*pharmacology
MH  - Macrophages, Peritoneal/*metabolism
MH  - Oxidation-Reduction
MH  - RNA, Messenger/metabolism
MH  - Rabbits
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0021-9150(97)00109-3 [pii]
AID - 10.1016/s0021-9150(97)00109-3 [doi]
PST - ppublish
SO  - Atherosclerosis. 1997 Aug;133(1):31-6. doi: 10.1016/s0021-9150(97)00109-3.