PMID- 9258664
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20071114
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 19
IP  - 4
DP  - 1997 Aug
TI  - Increased chromosome 20 copy number detected by fluorescence in situ 
      hybridization (FISH) in malignant melanoma.
PG  - 278-85
AB  - DNA amplification is an important mechanism of tumor progression that allows 
      cancer cells to up-regulate the expression of critical genes such as oncogenes 
      and genes conferring drug resistance. Recent studies using comparative genomic 
      hybridization (CGH) revealed increased DNA copies of 20q sequences in 7 melanoma 
      cell lines and B archival metastatic melanoma lesions. To evaluate chromosome 20 
      abnormalities in more detail and to resolve discrepancies between karyotype and 
      CGH findings, we performed FISH analysis of metaphase cells in 13 melanoma cell 
      lines (including the 7 lines used for CGH) and 9 primary melanoma specimens by 
      using a whole chromosome paint specific for chromosome 20. All 13 cell lines 
      (100%) and 8/9 primary tumors (89%) showed extra copies of chromosome 20 relative 
      to tumor ploidy. Additionally, 6/14 cell lines (43%) and 2/8 primary tumors (25%) 
      showed translocated chromosome 20 material previously undetected by standard 
      cytogenetics. Cytologic evidence for gene amplification was also found in one 
      cell line, which contained an add(20)(p13), with additional DNA being derived 
      from 20q sequences. These data suggest that overrepresentation of a gene or genes 
      important for melanoma pathogenesis resides on the long arm of chromosome 20.
FAU - Barks, J H
AU  - Barks JH
AD  - Graduate Interdisciplinary Program in Genetics, University of Arizona, Tucson, 
      USA.
FAU - Thompson, F H
AU  - Thompson FH
FAU - Taetle, R
AU  - Taetle R
FAU - Yang, J M
AU  - Yang JM
FAU - Stone, J F
AU  - Stone JF
FAU - Wymer, J A
AU  - Wymer JA
FAU - Khavari, R
AU  - Khavari R
FAU - Guan, X Y
AU  - Guan XY
FAU - Trent, J M
AU  - Trent JM
FAU - Pinkel, D
AU  - Pinkel D
FAU - Nelson, M A
AU  - Nelson MA
LA  - eng
GR  - 1R29 CA70145-01/CA/NCI NIH HHS/United States
GR  - CA23074/CA/NCI NIH HHS/United States
GR  - CA27502/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - Gene Amplification
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Melanoma/*genetics/pathology
MH  - Metaphase
MH  - Ploidies
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1997/08/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199708)19:4<278::AID-GCC11>3.0.CO;2-C [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Aug;19(4):278-85.