PMID- 9258875
OWN - NLM
STAT- MEDLINE
DCOM- 19970926
LR  - 20161124
IS  - 1071-5576 (Print)
IS  - 1071-5576 (Linking)
VI  - 4
IP  - 3
DP  - 1997 May-Jun
TI  - Effects of hypoxemia on 11 beta-hydroxysteroid dehydrogenase types 1 and 2 gene 
      expression in preterm fetal sheep.
PG  - 124-9
AB  - OBJECTIVE: To examine the effect of sustained hypoxia on the expression of 11 
      beta-hydroxysteroid dehydrogenase (11 beta-HSD) type 1 and 2 genes in preterm 
      fetal sheep. METHODS: Fetal liver and kidney as well as placental tissues were 
      collected at days 111-113 of gestation (term = 145 days) after 8 hours of 
      sustained hypoxemia induced by lowering the maternal inspired oxygen (n = 7) or 
      after 8 hours of normoxia to serve as controls (n = 5). Changes in the levels of 
      11 beta-HSD1 and 11 beta-HSD2 mRNA were determined by Northern blot analysis 
      using ovine 11 beta-HSD types 1 and 2 cDNAs as probes. Levels of 11 beta-HSD2 
      activity were determined by a standard radiometric conversion assay. RESULTS: In 
      hypoxic fetuses, there was a tendency for a decrease (P = .08) in levels of 11 
      beta-HSD2 mRNA in the kidney. This decrease was correlated significantly with the 
      degree of associated fetal acidemia (P < .01). However, there were no 
      corresponding changes in the level of renal 11 beta-HSD2 enzyme activity, 
      indicating that changes in 11 beta-HSD2 mRNA were unlikely carried through to 11 
      beta-HSD2 protein. In contrast levels of 11 beta-HSD1 mRNA in the placenta and 
      fetal liver were unchanged after sustained hypoxia. CONCLUSION: These results 
      demonstrate that fetal hypoxemia-induced acidosis selectively down-regulates 11 
      beta-HSD2 mRNA expression in the preterm fetal sheep kidney. This may provide a 
      further mechanism whereby fetal acidosis alters developmental processes by 
      regulating the bioavailability of glucocorticoids in specific fetal organs 
      through altered local expression of 11 beta-HSD enzymes.
FAU - Yang, K
AU  - Yang K
AD  - Lawson Research Institute, St. Joseph's Hospital, Department of Obstetrics and 
      Gynecology, London, Ontario, Canada.
FAU - Shearman, K
AU  - Shearman K
FAU - Asano, H
AU  - Asano H
FAU - Richardson, B S
AU  - Richardson BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Soc Gynecol Investig
JT  - Journal of the Society for Gynecologic Investigation
JID - 9433806
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Blood Gas Analysis
MH  - Embryonic and Fetal Development/genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Gestational Age
MH  - Hydrogen-Ion Concentration
MH  - Hydroxysteroid Dehydrogenases/*genetics
MH  - Hypoxia/*metabolism
MH  - Kidney/embryology/metabolism
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Sheep
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S1071557697000130 [pii]
PST - ppublish
SO  - J Soc Gynecol Investig. 1997 May-Jun;4(3):124-9.