PMID- 9258910
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20071114
IS  - 0969-9961 (Print)
IS  - 0969-9961 (Linking)
VI  - 4
IP  - 1
DP  - 1997
TI  - Cellular distribution of NMDA glutamate receptor subunit mRNAs in the human 
      cerebellum.
PG  - 35-46
AB  - We have used a quantitative in situ hybridization method with human 
      ribonucleotide probes to examine the regional and cellular distribution of 
      N-methyl-D-aspartate receptor (NMDAR) subunit mRNAs in the human cerebellum. 
      Purkinje cells showed very dense labeling for NMDAR1 mRNA, dense labeling for 
      NMDAR2A mRNA, and moderate labeling for NMDAR2D mRNA, whereas labeling for 
      NMDAR2C mRNA was low. Granule cells showed high hybridization signals for the 
      NMDAR1 and NMDAR2C mRNAs and moderate signals for the NMDAR2A and NMDAR2D mRNAs. 
      In addition intense labeling with the NMDAR2B probe was observed in medium-sized 
      neurons with chromophilic cell bodies in the upper part of the granule cell 
      layer, most likely representing Golgi cells. Neurons in the molecular layer, 
      i.e., basket cells and stellate cells, showed moderate hybridization signals for 
      NMDAR1 and NMDAR2D and low signal for NMDAR2C. Each type of cerebellar neuron 
      analyzed displayed a distinct NMDAR2 subunit profile, suggesting that they are 
      likely to have NMDA receptors with distinct properties.
FAU - Scherzer, C R
AU  - Scherzer CR
AD  - Department of Neurology, Massachusetts General Hospital, Boston 02114, USA.
FAU - Landwehrmeyer, G B
AU  - Landwehrmeyer GB
FAU - Kerner, J A
AU  - Kerner JA
FAU - Standaert, D G
AU  - Standaert DG
FAU - Hollingsworth, Z R
AU  - Hollingsworth ZR
FAU - Daggett, L P
AU  - Daggett LP
FAU - Velicelebi, G
AU  - Velicelebi G
FAU - Penney, J B Jr
AU  - Penney JB Jr
FAU - Young, A B
AU  - Young AB
LA  - eng
SI  - GENBANK/U77782
SI  - GENBANK/U77783
GR  - AG11337/AG/NIA NIH HHS/United States
GR  - MH/NS 31862/MH/NIMH NIH HHS/United States
GR  - NS31579/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Macromolecular Substances)
RN  - 0 (NMDA receptor A1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Cerebellar Cortex/metabolism
MH  - Cerebellum/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Macromolecular Substances
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Receptors, N-Methyl-D-Aspartate/*biosynthesis/*chemistry
EDAT- 1997/01/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S0969-9961(97)90136-4 [pii]
AID - 10.1006/nbdi.1997.0136 [doi]
PST - ppublish
SO  - Neurobiol Dis. 1997;4(1):35-46. doi: 10.1006/nbdi.1997.0136.