PMID- 9259186
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20131121
IS  - 1018-7782 (Print)
IS  - 1018-7782 (Linking)
VI  - 5
IP  - 4
DP  - 1997 Jul-Aug
TI  - Production of monocyte chemoattractant protein-1 by cultured glomerular 
      epithelial cells: inhibition by dexamethasone.
PG  - 318-22
AB  - Glomerular epithelial cells (GECs) have been shown to be one class of target 
      cells in immunological and nonimmunological glomerular injury of 
      glomerulonephritis, some cases of which are accompanied by infiltration of 
      glomeruli by monocytes/macrophages. In this study we tested whether GECs in 
      culture produce monocyte chemoattractant protein-1 (MCP-1), a potential molecule 
      responsible for monocyte recruitment in inflammation, and whether the production 
      is inhibited by glucocorticoid. GECs were obtained from outgrowth of rat 
      glomeruli. Levels of MCP-1 mRNA and protein were determined by Northern blot 
      analysis and ELISA, respectively. Northern blot analysis revealed the expression 
      of MCP-1 mRNA in cytokine-treated GECs. The expression of MCP-1 mRNA was 
      inhibited by dexamethasone. Quantitative analysis by ELISA confirmed the 
      production of MCP-1 protein by GECs and the inhibitory effect of dexamethasone. 
      These results indicate that cytokine-treated GECs produce and secrete MCP-1 and 
      that the MCP-1 production is inhibited by dexamethasone. We suggest that MCP-1 
      produced by GECs may play a role in the recruitment of monocytes/macrophages in 
      glomerulonephritis and that the therapeutic effect glucocorticoid on the disease 
      might include the inhibition of the production of MCP-1.
FAU - Natori, Y
AU  - Natori Y
AD  - Research Institute, International Medical Center of Japan, Tokyo, Japan. 
      natoriya@imcj.go.jp
FAU - Natori, Y
AU  - Natori Y
FAU - Nishimura, T
AU  - Nishimura T
FAU - Yamabe, H
AU  - Yamabe H
FAU - Iyonaga, K
AU  - Iyonaga K
FAU - Takeya, M
AU  - Takeya M
FAU - Kawakami, M
AU  - Kawakami M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Exp Nephrol
JT  - Experimental nephrology
JID - 9302239
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/physiology
MH  - Chemotaxis/drug effects
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Culture Media, Conditioned
MH  - Cytokines/*pharmacology
MH  - Dexamethasone/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells
MH  - Epithelium/drug effects/immunology
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Kidney Glomerulus/*immunology
MH  - Leukocytes, Mononuclear/physiology
MH  - Macrophages, Peritoneal/drug effects/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Proteins/pharmacology
MH  - Transcription, Genetic/*drug effects
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
PST - ppublish
SO  - Exp Nephrol. 1997 Jul-Aug;5(4):318-22.