PMID- 9261403
OWN - NLM
STAT- MEDLINE
DCOM- 19970917
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 71
IP  - 9
DP  - 1997 Sep
TI  - Analysis of bovine herpesvirus 1 transcripts during a primary infection of 
      trigeminal ganglia of cattle.
PG  - 6786-95
AB  - During an infection of nonneuronal cells, bovine herpesvirus 1 (BHV-1) gene 
      expression proceeds in a well-defined cascade. Products of immediate-early (IE) 
      genes are expressed first, and they activate expression of early (E) and late (L) 
      genes. Although the same cascade is assumed to occur during an infection of 
      neurons in trigeminal ganglia (TG) of cattle, no experimental data is available 
      to support this hypothesis. Consequently, we analyzed BHV-1 gene expression in 
      bovine TG at 1, 2, 4, 7, and 15 days postinfection (dpi). Infectious virus was 
      detected in ocular swabs from 1 to 7 dpi but not 15 dpi. By reverse transcription 
      (RT)-PCR, IE (bICP4), E (thymidine kinase, ribonucleotide reductase [RR]), L 
      (glycoprotein C, and alpha trans-inducing factor), and dual-kinetic (bICP0 and 
      bICP22) transcripts were analyzed. When cDNA synthesis was primed with random 
      hexamers, IE and E transcripts were detected at the same time. However, 
      full-length and poly(A)+ (FL&P) RR or bICP22 RNAs were detected before FL&P IE 
      RNAs. Furthermore, FL&P IE transcripts were not detected until viral DNA 
      increased in TG. IE transcripts were detected before E or L RNAs when rabbit 
      kidney cells were infected with a low multiplicity of infection and the same 
      RT-PCR detection method was used. These studies suggested that expression of 
      full-length and polyadenylated IE transcripts in trigeminal ganglia was not 
      efficient compared to that of RR and bICP22 transcripts.
FAU - Schang, L M
AU  - Schang LM
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln 
      68583-0905, USA.
FAU - Jones, C
AU  - Jones C
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*virology
MH  - Cell Line
MH  - Cricetinae
MH  - DNA Primers
MH  - DNA, Viral
MH  - Genes, Immediate-Early
MH  - Genes, Viral
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Herpesvirus 1, Bovine/*genetics
MH  - Poly A/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/metabolism
MH  - Rabbits
MH  - Trigeminal Ganglion/*virology
PMC - PMC191959
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
PMCR- 1997/09/01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
PHST- 1997/09/01 00:00 [pmc-release]
AID - 10.1128/JVI.71.9.6786-6795.1997 [doi]
PST - ppublish
SO  - J Virol. 1997 Sep;71(9):6786-95. doi: 10.1128/JVI.71.9.6786-6795.1997.