PMID- 9263395
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 87
IP  - 3
DP  - 1997 Aug 1
TI  - Alterations in the free radical generation and nitric oxide release from rat 
      peripheral polymorphonuclear leukocytes following thrombosis.
PG  - 279-88
AB  - The present investigation was undertaken to study the alterations in free radical 
      generation and release of nitric oxide (NO) from polymorphonuclear leukocytes 
      (PMNLs) following thrombosis. Thrombosis was induced in rats by intravenous 
      injection of collagen and adrenaline. PMNLs were separated from rat blood by 
      using dextran sedimentation and Ficoll-Hypaque. Arachidonic acid (AA), formyl 
      methionine leucine phenylalanine (FMLP) and opsonized zymosan (OZ) induced free 
      radical generation was estimated as luminol (LCL) and Lucigenin (LUCDCL) 
      dependent chemiluminescence. PMNLs nitric oxide synthase (NOS) activity and NO 
      release were measured by using [14C] L-Arginine (L-Arg) and oxy-hemoglobin 
      respectively. LCL and LUCDCL responses in rat PMNLs were significantly attenuated 
      following thrombosis. There was no change in the release of myeloperoxidase 
      enzyme (MPO) from PMNLs obtained following thrombosis. PMNLs NOS activity and NO 
      release were also found to be increased after thrombosis. Pretreatment of rat 
      PMNLs with 10 mM L-NAME (NO precursor) or 100 microM sodium nitroprusside (NO 
      donor), resulted in significant reduction of AA induced LCL response. Results 
      obtained indicate that NO release form PMNLs was augmented while free radical 
      generation response was attenuated after the induction of thrombosis.
FAU - Seth, P
AU  - Seth P
AD  - Division of Pharmacology, Central Drug Research Institute, Lucknow, India.
FAU - Kumari, R
AU  - Kumari R
FAU - Dikshit, M
AU  - Dikshit M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Free Radicals)
RN  - 169D1260KM (Nitroprusside)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9007-34-5 (Collagen)
RN  - 9010-72-4 (Zymosan)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/pharmacology
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Collagen/toxicity
MH  - Epinephrine/toxicity
MH  - Free Radicals
MH  - Luminescent Measurements
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Neutrophils/*metabolism
MH  - Nitric Oxide/*blood
MH  - Nitric Oxide Synthase/metabolism
MH  - Nitroprusside/pharmacology
MH  - Peroxidase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thrombosis/*blood/chemically induced
MH  - Zymosan/pharmacology
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0049-3848(97)00130-8 [pii]
AID - 10.1016/s0049-3848(97)00130-8 [doi]
PST - ppublish
SO  - Thromb Res. 1997 Aug 1;87(3):279-88. doi: 10.1016/s0049-3848(97)00130-8.