PMID- 9263450
OWN - NLM
STAT- MEDLINE
DCOM- 19971006
LR  - 20190905
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 12
IP  - 1
DP  - 1997 Jul
TI  - Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by 
      multi-color fluorescence in situ hybridization with cosmid clones.
PG  - 31-7
AB  - The 5S ribosomal RNA genes were mapped to mitotic chromosomes of Arabidopsis 
      thaliana by fluorescence in situ hybridization (FISH). In the ecotype Landsberg 
      erecta, hybridization signals appeared on three pairs of chromosomes, two of 
      which were metacentric and the other acrocentric. Hybridization signals on one 
      pair of metacentric chromosomes were much stronger than those on the acrocentric 
      and the other pair of metacentric chromosomes, probably reflecting the number of 
      copies of the genes on the chromosomes. Other ecotypes, Columbia and 
      Wassilewskija, had similar chromosomal distribution of the genes, but the 
      hybridization signals on one pair of metacentric chromosomes were very weak, and 
      detectable only in chromosomes prepared from young flower buds. The chromosomes 
      and arms carrying the 5S rDNA were identified by multi-color FISH with cosmid 
      clones and a centromeric 180 bp repeat as co-probes. The metacentric chromosome 5 
      and its L arm carries the largest cluster of the genes, and the short arm of 
      acrocentric chromosome 4 carries a small cluster in all three ecotypes. 
      Chromosome 3 had another small cluster of 5S rRNA genes on its L arm. Chromosome 
      1 and 2 had no 5S rDNA cluster, but they are morphologically distinguishable; 
      chromosome 1 is metacentric and 2 acrocentric. Using the 5S rDNA as a probe, 
      therefore, all chromosomes of A. thaliana could be identified by FISH. Chromosome 
      1 is large and metacentric; chromosome 2 is acrocentric carrying 18S-5.8S-25S 
      rDNA clusters on its short arm; chromosome 3 is metacentric carrying a small 
      cluster of 5S rDNA genes on its L arm; chromosome 4 is acrocentric carrying both 
      18S-5.8S-25S and 5S rDNAs on its short (L) arm; and/chromosome 5 is metacentric 
      carrying a large cluster of 5S rDNA on its L arm.
FAU - Murata, M
AU  - Murata M
AD  - Research Institute for Bioresources, Okayama University, Kurashiki, Japan. 
      mmura@rib.okayama-u.ac.jp
FAU - Heslop-Harrison, J S
AU  - Heslop-Harrison JS
FAU - Motoyoshi, F
AU  - Motoyoshi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Arabidopsis/*genetics/physiology
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Cosmids
MH  - DNA, Ribosomal/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Multigene Family
MH  - RNA, Plant/genetics
MH  - RNA, Ribosomal, 5S/biosynthesis/*genetics
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1046/j.1365-313x.1997.12010031.x [doi]
PST - ppublish
SO  - Plant J. 1997 Jul;12(1):31-7. doi: 10.1046/j.1365-313x.1997.12010031.x.