PMID- 9263916
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 502 ( Pt 2)
IP  - Pt 2
DP  - 1997 Jul 15
TI  - Calcium release from intracellular stores evoked by extracellular ATP in a 
      Xenopus renal epithelial cell line.
PG  - 365-73
AB  - 1. The signal transduction mechanism mediating extracellular adenosine 
      5'-triphosphate (ATP)-induced calcium release in a renal epithelial cell line 
      (A6) was investigated using the whole-cell voltage-clamp technique and fura-2 
      fluorescence measurement. 2. ATP (10 microM) activated calcium-dependent 
      non-selective cation channels in cells held under voltage clamp. 3. Guanosine 
      5'-O-(2-thiodiphosphate) (GDP beta S; 0.1-1.0 mM) in the pipette inhibited the 
      ATP-activated calcium-dependent currents. With guanosine 
      5'-O-(3-thiotriphosphate) (GTP gamma S; 0.1-1.0 mM) in the pipette, the currents 
      were spontaneously elicited without application of ATP. Pretreatment with 
      pertussis toxin (PTX) affected neither the ATP-activated currents nor the 
      increase in intracellular free calcium concentration ([Ca2+]i) evoked by ATP. 4. 
      Intracellular application of neomycin or heparin inhibited the ATP-activated 
      currents. Inositol 1,4,5-trisphosphate (IP3; 0.1-100 microM) in the internal 
      solution produced currents similar to those due to ATP activation. 5. These 
      results suggest that a PTX-insensitive guanosine 5'-triphosphate (GTP)-binding 
      regulatory protein (G. protein) is involved in extracellular. ATP-induced 
      phosphoinositide turnover and subsequent calcium release from IP3-sensitive 
      stores, which subsequently activates the calcium-dependent channels in A6 cells.
FAU - Mori, M
AU  - Mori M
AD  - Department of Physiology, Kobe University, School of Medicine, Japan. 
      mm@med.kobe-u.ac.jp
FAU - Hosomi, H
AU  - Hosomi H
FAU - Nishizaki, T
AU  - Nishizaki T
FAU - Kawahara, K
AU  - Kawahara K
FAU - Okada, Y
AU  - Okada Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Thionucleotides)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 526U7A2651 (Egtazic Acid)
RN  - 71376-97-1 (guanosine 5'-O-(2-thiodiphosphate))
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9005-49-6 (Heparin)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - I16QD7X297 (Neomycin)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Egtazic Acid/pharmacology
MH  - Epithelium/drug effects/physiology
MH  - Fura-2
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Diphosphate/analogs & derivatives/pharmacology
MH  - Heparin/pharmacology
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - Kidney/*physiology
MH  - Kinetics
MH  - Membrane Potentials/drug effects/physiology
MH  - Microscopy, Fluorescence
MH  - Neomycin/pharmacology
MH  - Patch-Clamp Techniques
MH  - Pertussis Toxin
MH  - Signal Transduction/drug effects
MH  - Thionucleotides/pharmacology
MH  - Virulence Factors, Bordetella/pharmacology
MH  - Xenopus
PMC - PMC1159555
EDAT- 1997/07/15 00:00
MHDA- 1997/07/15 00:01
PMCR- 1997/07/15
CRDT- 1997/07/15 00:00
PHST- 1997/07/15 00:00 [pubmed]
PHST- 1997/07/15 00:01 [medline]
PHST- 1997/07/15 00:00 [entrez]
PHST- 1997/07/15 00:00 [pmc-release]
AID - 10.1111/j.1469-7793.1997.365bk.x [doi]
PST - ppublish
SO  - J Physiol. 1997 Jul 15;502 ( Pt 2)(Pt 2):365-73. doi: 
      10.1111/j.1469-7793.1997.365bk.x.