PMID- 9266840
OWN - NLM
STAT- MEDLINE
DCOM- 19970908
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 237
IP  - 1
DP  - 1997 Aug 8
TI  - Ca2+-ATPase inhibitors and Ca2+-ionophore induce osteoclast-like cell formation 
      in the cocultures of mouse bone marrow cells and calvarial cells.
PG  - 111-5
AB  - Osteoclasts which derive from hemopoietic cells are multinucleated cells 
      responsible for bone resorption. We found that cyclopiazonic acid (CPA), 
      thapsigargin (TG), and 2,5-di-(t-butyl)-1,4-hydroquinone (BHQ) induced 
      osteoclast-like cell (OCL) formation in cocultures of mouse calvaria-derived 
      stromal cells and hemopoietic cells such as bone marrow cells and spleen cells. 
      OCLs induced by these compounds showed typical characteristics of osteoclasts 
      such as tartrate-resistant acid phosphatase activity and pit forming activity. 
      These compounds are known as endoplasmic reticulum (ER)/sarcoplasmic reticulum 
      (SR) Ca2+-ATPase inhibitors that increase intracellular Ca2+ levels by inhibiting 
      Ca2+-ATPase activity located in the membrane of ER/SR. Ca2+-ionophores such as 
      ionomycin which increase intracellular Ca2+ levels also stimulated OCL formation 
      in the cocultures. Differentiation of hemopoietic cells into OCLs induced by 
      these compounds required the presence of calvarial cells. These results indicate 
      that an increase of intracellular Ca2+ levels may be a part of signaling pathways 
      to induce osteoclast differentiation in the presence of calvarial cells.
FAU - Takami, M
AU  - Takami M
AD  - Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
FAU - Woo, J T
AU  - Woo JT
FAU - Takahashi, N
AU  - Takahashi N
FAU - Suda, T
AU  - Suda T
FAU - Nagai, K
AU  - Nagai K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydroquinones)
RN  - 0 (Indoles)
RN  - 0 (Ionophores)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - 56092-81-0 (Ionomycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/*antagonists & inhibitors
MH  - Cell Differentiation/drug effects
MH  - Coated Pits, Cell-Membrane/drug effects/ultrastructure
MH  - Coculture Techniques
MH  - Enzyme Inhibitors/*pharmacology
MH  - Hematopoietic Stem Cells/*cytology/drug effects
MH  - Hydroquinones/pharmacology
MH  - Indoles/pharmacology
MH  - Ionomycin/*pharmacology
MH  - Ionophores/pharmacology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Osteoclasts/*cytology/drug effects
MH  - Skull/*cytology/drug effects
MH  - Thapsigargin/pharmacology
EDAT- 1997/08/08 00:00
MHDA- 1997/08/08 00:01
CRDT- 1997/08/08 00:00
PHST- 1997/08/08 00:00 [pubmed]
PHST- 1997/08/08 00:01 [medline]
PHST- 1997/08/08 00:00 [entrez]
AID - S0006-291X(97)97090-X [pii]
AID - 10.1006/bbrc.1997.7090 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 Aug 8;237(1):111-5. doi: 10.1006/bbrc.1997.7090.