PMID- 9266843
OWN - NLM
STAT- MEDLINE
DCOM- 19970908
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 237
IP  - 1
DP  - 1997 Aug 8
TI  - Interleukin-18 induces activation and association of p56(lck) and MAPK in a 
      murine TH1 clone.
PG  - 126-30
AB  - Interleukin-18 (IL-18) was identified as an inducer of interferon-gamma 
      (IFN-gamma) production by stimulated T cells. In this study, we used an 
      ovalbumin-responsive murine Th1 clone (OVA#4), in which DNA synthesis was 
      reportedly enhanced after IL-18 treatment in the presence of a non-mitogenic 
      TCR/CD3 stimulus, to examine signal transduction pathways. In the presence of the 
      stimulus, IL-18 induced the appearance of tyrosine-phosphorylated proteins and 
      herbimycin A inhibited DNA synthesis. It is suggested that protein tyrosine 
      kinase (PTK) mediated signaling is induced by IL-18. Specifically, IL-18 induced 
      phosphorylation of phosphorylates p56(lck) (LCK) and mitogen-activated protein 
      kinase (MAPK). IL-18 alone induced the kinase activities of both LCK and MAPK, 
      and the activities were increased by the TCR/CD3 stimulus. Simultaneously, IL-18 
      induced the association of LCK with MAPK and this was also increased by the 
      TCR/CD3 stimulus. The activation of the LCK-MAPK pathway correlated with enhanced 
      DNA synthesis in OVA#4 cells. These results suggest that the LCK-MAPK pathway is 
      involved in IL-18 signaling and that IL-18 may play an important role in 
      modification of TCR/CD3-mediated response.
FAU - Tsuji-Takayama, K
AU  - Tsuji-Takayama K
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan. 
      fujih@po.harenet.or.jp
FAU - Matsumoto, S
AU  - Matsumoto S
FAU - Koide, K
AU  - Koide K
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Ikeda, M
AU  - Ikeda M
FAU - Ohta, T
AU  - Ohta T
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Benzoquinones)
RN  - 0 (CD3 Complex)
RN  - 0 (Cytokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Interleukin-18)
RN  - 0 (Lactams, Macrocyclic)
RN  - 0 (Quinones)
RN  - 1W306TDA6S (Rifabutin)
RN  - 70563-58-5 (herbimycin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck))
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Benzoquinones
MH  - CD3 Complex/immunology/physiology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Clone Cells
MH  - Cytokines/*pharmacology
MH  - DNA Replication/drug effects
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Interleukin-18
MH  - Kinetics
MH  - Lactams, Macrocyclic
MH  - Lymphocyte Activation
MH  - Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
MH  - Mice
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/metabolism
MH  - Quinones/pharmacology
MH  - Rifabutin/analogs & derivatives
MH  - Th1 Cells/drug effects/immunology/*physiology
MH  - Time Factors
MH  - src-Family Kinases/*metabolism
EDAT- 1997/08/08 00:00
MHDA- 1997/08/08 00:01
CRDT- 1997/08/08 00:00
PHST- 1997/08/08 00:00 [pubmed]
PHST- 1997/08/08 00:01 [medline]
PHST- 1997/08/08 00:00 [entrez]
AID - S0006-291X(97)97099-6 [pii]
AID - 10.1006/bbrc.1997.7099 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 Aug 8;237(1):126-30. doi: 
      10.1006/bbrc.1997.7099.