PMID- 9266879
OWN - NLM
STAT- MEDLINE
DCOM- 19970909
LR  - 20190514
IS  - 0012-3692 (Print)
IS  - 0012-3692 (Linking)
VI  - 112
IP  - 2
DP  - 1997 Aug
TI  - Origin of pleural cells after lung transplantation: from donor or recipient?
PG  - 426-9
AB  - We evaluated the change in the percentage of cells of donor origin in pleural 
      fluid of 13 consecutive patients who underwent lung transplantation. Pleural 
      fluid was sampled 2, 4, and 8 days after lung transplantation. DNA, which was 
      extracted from the blood of donors and recipients and from the pleural fluid, was 
      amplified using a polymerase chain reaction technique. The reaction products were 
      electrophoresed, and bands indicating amplified human leukocyte antigen (HLA)-DR 
      alleles were quantified by determining the area under the curve (AUC) by a 
      densitometric analysis. HLA-DR alleles, which were present only in recipient 
      cells (recipient allele), were analyzed and compared to HLA-DR alleles that were 
      present only in donor cells (donor allele). A dilution study was first performed 
      to provide a standard curve relating the percentage of donor and recipient cells 
      in a mixture to their AUC. The AUC of the recipient alleles did not change 
      significantly over the first 8 postoperative days. The AUC of the donor alleles 
      was less on postoperative days 4 and 8 than on day 2 (p<0.05). The donor allele 
      AUC on day 8 was <20% of the shared allele AUC, corresponding to <1% of all cells 
      by the dilution study. We conclude that donor cells are rapidly cleared from the 
      pleural space after lung transplantation, with <1% of cells of donor origin by 
      postoperative day 8.
FAU - Judson, M A
AU  - Judson MA
AD  - Division of Pulmonary and Critical Care Medicine, Medical University of South 
      Carolina, Charleston 29425-2220, USA.
FAU - Sahn, S A
AU  - Sahn SA
FAU - Hahn, A B
AU  - Hahn AB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Chest
JT  - Chest
JID - 0231335
RN  - 0 (HLA-DR Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Cell Movement
MH  - DNA/analysis
MH  - Female
MH  - HLA-DR Antigens/genetics
MH  - Heart-Lung Transplantation/pathology
MH  - Humans
MH  - Lung Transplantation/*pathology
MH  - Male
MH  - Middle Aged
MH  - Pleura/*cytology
MH  - Pleural Effusion/*cytology
MH  - Polymerase Chain Reaction
MH  - Postoperative Period
MH  - Time Factors
MH  - Tissue Donors
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0012-3692(15)53012-2 [pii]
AID - 10.1378/chest.112.2.426 [doi]
PST - ppublish
SO  - Chest. 1997 Aug;112(2):426-9. doi: 10.1378/chest.112.2.426.