PMID- 9267014
OWN - NLM
STAT- MEDLINE
DCOM- 19970908
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 78 ( Pt 8)
DP  - 1997 Aug
TI  - Evidence that DNA-A of a geminivirus associated with severe cassava mosaic 
      disease in Uganda has arisen by interspecific recombination.
PG  - 2101-11
AB  - Geminivirus isolates associated with the epidemic of severe cassava mosaic 
      disease in Uganda were studied and compared with virus isolates from the part of 
      Uganda outside the epidemic area, and with African cassava mosaic virus (ACMV) 
      and East African cassava mosaic virus (EACMV). Isolates of a novel type [the 
      Uganda variant (UgV)] were detected in severely affected plants from the epidemic 
      area, whereas those from plants outside the epidemic area were typical of ACMV. 
      The complete nucleotide sequences of DNA-A of UgV (2799 nt) and of a Tanzanian 
      isolate of EACMV (2801 nt) were determined and are extremely similar, except for 
      the coat protein (CP) gene. The CP gene of UgV has three distinct regions: the 5' 
      219 nt are 99% identical to EACMV (only 79% to ACMV); the following 459 nt are 
      99% identical to ACMV (75% to EACMV); and the 3' 93 nt are 98% identical to EACMV 
      (76% to ACMV). UgV DNA-A therefore is considered to have arisen by interspecific 
      recombination of EACMV and ACMV. Despite the hybrid nature of their CP, UgV 
      isolates were indistinguishable from ACMV in tests with 20 monoclonal antibodies 
      (MAbs), including seven which reacted with ACMV but not EACMV. The discontinuous 
      epitopes detected by these seven MAbs must involve amino acids which lie in the 
      central part of the CP (residues 74-226) and which differ in ACMV and EACMV. UgV 
      isolates were detected in severely mosaic-affected plants from all 11 widely 
      separated locations sampled. The probable role of recombination in geminivirus 
      evolution in the short to medium term is discussed.
FAU - Zhou, X
AU  - Zhou X
AD  - Scottish Crop Research Institute, Invergowrie, Dundee, UK.
FAU - Liu, Y
AU  - Liu Y
FAU - Calvert, L
AU  - Calvert L
FAU - Munoz, C
AU  - Munoz C
FAU - Otim-Nape, G W
AU  - Otim-Nape GW
FAU - Robinson, D J
AU  - Robinson DJ
FAU - Harrison, B D
AU  - Harrison BD
LA  - eng
SI  - GENBANK/Z83252
SI  - GENBANK/Z83253
SI  - GENBANK/Z83254
SI  - GENBANK/Z83255
SI  - GENBANK/Z83256
SI  - GENBANK/Z83257
SI  - GENBANK/Z83258
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Capsid/genetics
MH  - DNA Primers
MH  - DNA, Viral/*chemistry/genetics
MH  - Geminiviridae/genetics/isolation & purification/*pathogenicity
MH  - Introns
MH  - Manihot/*virology
MH  - Molecular Sequence Data
MH  - Plant Diseases/*virology
MH  - Polymerase Chain Reaction
MH  - *Recombination, Genetic
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Uganda
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1099/0022-1317-78-8-2101 [doi]
PST - ppublish
SO  - J Gen Virol. 1997 Aug;78 ( Pt 8):2101-11. doi: 10.1099/0022-1317-78-8-2101.