PMID- 9267898
OWN - NLM
STAT- MEDLINE
DCOM- 19971002
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 17
IP  - 8
DP  - 1997 Aug
TI  - The proportion of fetal nucleated red blood cells in maternal blood: estimation 
      by FACS analysis.
PG  - 743-52
AB  - The purpose of this study was to determine the proportion of fetal nucleated red 
      blood cells (NRBCs) among enriched NRBCs and to evaluate the effectiveness of 
      enriching NRBCs in maternal blood using fluorescence-activated cell sorting 
      (FACS) to separate NRBCs. The origin of enriched NRBCs was determined using 
      fluorescence in situ hybridization (FISH) methods. Y-specific signals were 
      observed in 4.6 +/- 1.5 per cent of the enriched cells from 14 of 16 (87.5 per 
      cent) pregnant women who gave birth to boys. In this series, the specificity of 
      the fetal sex diagnosis was 100 per cent, the sensitivity 88 per cent, and the 
      negative predictive value 86 per cent. Fetal NRBCs are present in maternal blood 
      and FACS has the potential to enrich fetal NRBCs. Fetal cells were estimated to 
      be enriched more than 10,000-fold in the first trimester and more than 100-fold 
      in the third trimester. Average frequencies of fetal cells in maternal blood were 
      8.1 x 10(-5) and 1.6 x 10(-5) in the first trimester and the second/third 
      trimesters. However, most of the NRBCs in maternal blood are maternal in origin.
FAU - Sohda, S
AU  - Sohda S
AD  - Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan. 
      ssohda@md.tsukuba.ac.jp
FAU - Arinami, T
AU  - Arinami T
FAU - Hamada, H
AU  - Hamada H
FAU - Nakauchi, H
AU  - Nakauchi H
FAU - Hamaguchi, H
AU  - Hamaguchi H
FAU - Kubo, T
AU  - Kubo T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (CD36 Antigens)
RN  - 0 (CD71 antigen)
RN  - 0 (Glycophorins)
RN  - 0 (Receptors, Transferrin)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, B-Lymphocyte/analysis
MH  - CD36 Antigens/analysis
MH  - Cell Nucleus
MH  - *Erythrocyte Count
MH  - Erythrocytes/*ultrastructure
MH  - Female
MH  - Fetal Blood/*cytology
MH  - *Flow Cytometry
MH  - Glycophorins/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Receptors, Transferrin
EDAT- 1997/08/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0223(199708)17:8<743::AID-PD144>3.0.CO;2-3 [pii]
PST - ppublish
SO  - Prenat Diagn. 1997 Aug;17(8):743-52.