PMID- 9268170
OWN - NLM
STAT- MEDLINE
DCOM- 19970909
LR  - 20171116
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 234
IP  - 2
DP  - 1997 Aug 4
TI  - Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T 
      lymphocyte clones.
PG  - 383-6
AB  - We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte 
      (CTL) clones for protein recognition, using recombinant vaccinia viruses which 
      contain genes coding for dengue virus proteins. These clones were established 
      from peripheral blood mononuclear cells of a donor previously immunized with a 
      live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven 
      were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four 
      dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone 
      recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte 
      antigen (HLA) restriction revealed that three dengue-1-specific clones are 
      HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is 
      HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as 
      C, E, and NS3 proteins reported earlier contain one or more epitopes recognized 
      by dengue virus-specific human CD4+ T lymphocytes.
FAU - Green, S
AU  - Green S
AD  - Department of Medicine, University of Massachusetts Medical Center, Worcester 
      01655, USA.
FAU - Kurane, I
AU  - Kurane I
FAU - Pincus, S
AU  - Pincus S
FAU - Paoletti, E
AU  - Paoletti E
FAU - Ennis, F A
AU  - Ennis FA
LA  - eng
GR  - NIH-R01-AI 30624/AI/NIAID NIH HHS/United States
GR  - T32-AI 07272/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, Viral)
RN  - 0 (CD4 Antigens)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - CD4 Antigens
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Clone Cells
MH  - *Cytotoxicity, Immunologic
MH  - Dengue/*immunology
MH  - Dengue Virus/*immunology
MH  - Humans
MH  - Viral Nonstructural Proteins/*immunology
EDAT- 1997/08/04 00:00
MHDA- 1997/08/04 00:01
CRDT- 1997/08/04 00:00
PHST- 1997/08/04 00:00 [pubmed]
PHST- 1997/08/04 00:01 [medline]
PHST- 1997/08/04 00:00 [entrez]
AID - S0042-6822(97)98648-7 [pii]
AID - 10.1006/viro.1997.8648 [doi]
PST - ppublish
SO  - Virology. 1997 Aug 4;234(2):383-6. doi: 10.1006/viro.1997.8648.