PMID- 9269328
OWN - NLM
STAT- MEDLINE
DCOM- 19970910
LR  - 20220311
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 392
IP  - 1-2
DP  - 1997 Aug 1
TI  - The in vitro micronucleus test: a multi-endpoint assay to detect simultaneously 
      mitotic delay, apoptosis, chromosome breakage, chromosome loss and 
      non-disjunction.
PG  - 19-30
AB  - Genotoxicity testing aims to detect a large range of genetic damage endpoints and 
      evaluate such results in context of cell survival. The cytokinesis block 
      micronucleus test offers the advantage to provide simultaneously information on 
      both cell cycle progression and chromosome/genome mutations. Indeed, 1. 
      frequencies of cytokinesis-blocked binucleated cells (and polynucleated) are good 
      estimators of the mitotic rate; 2. frequencies of apoptotic figures in 
      mononucleated and binucleated cells provide a measure for cell death before or 
      after cell division; 3. combination of fluorescence in situ hybridization (FISH) 
      for centromere/telomeres and micronucleus scoring allows discrimination between 
      clastogenic and aneugenic events; 4. detection of FISH signals for chromosome 
      specific sequences in both macronuclei and micronuclei, discriminates between 
      aneuploidy due to chromosome non-disjunction or to chromosome loss. The 
      cytokinesis block in vitro micronucleus test is thus a cytogenetic multi-test 
      providing mechanistic information with a simple, rapid, objective, microscopical 
      analysis.
FAU - Kirsch-Volders, M
AU  - Kirsch-Volders M
AD  - Laboratory for Anthropogenetics, Vrije Universiteit Brussel, Belgium.
FAU - Elhajouji, A
AU  - Elhajouji A
FAU - Cundari, E
AU  - Cundari E
FAU - Van Hummelen, P
AU  - Van Hummelen P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Apoptosis
MH  - Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Micronucleus Tests/*methods
MH  - Mitosis
RF  - 79
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0165-1218(97)00042-6 [pii]
AID - 10.1016/s0165-1218(97)00042-6 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Aug 1;392(1-2):19-30. doi: 10.1016/s0165-1218(97)00042-6.