PMID- 9269333
OWN - NLM
STAT- MEDLINE
DCOM- 19970910
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 392
IP  - 1-2
DP  - 1997 Aug 1
TI  - A critical evaluation of centromeric labeling to distinguish micronuclei induced 
      by chromosomal loss and breakage in vitro.
PG  - 81-95
AB  - The in vitro micronucleus assay in conjunction with CREST-staining and 
      fluorescence in situ hybridization (FISH) with centromere-specific DNA probes is 
      being increasingly utilized for the detection of clastogenic and 
      aneuploidy-inducing agents. Although potentially powerful techniques, both 
      methods have unique characteristics that can influence sample processing and the 
      interpretation of results. In this article, the use of the CREST and the FISH 
      modifications of the in vitro micronucleus assay have been used to characterize 
      the origin of the micronuclei induced by cyclophosphamide, 
      4,4'-methylene-bis(2-chloroaniline), 4-nitroquinoline N-oxide and ionizing 
      radiation in metabolically competent MCL-5 cells or a derived cell line lacking 
      metabolic activation. Using these results and our previous experiences with these 
      techniques, a detailed comparison including the strengths and limitations of each 
      technique as well as potential problems in performing each assay and in analyzing 
      the data is discussed. In spite of their limitations, our results to date 
      indicate that CREST-staining as well as FISH with centromere-specific DNA probes 
      can be used to accurately distinguish micronuclei formed from chromosome loss 
      from those originating from chromosome breakage and that these techniques can be 
      valuable complements to the in vitro micronucleus assay.
FAU - Schuler, M
AU  - Schuler M
AD  - Department of Entomology, University of California, Riverside 92521, USA.
FAU - Rupa, D S
AU  - Rupa DS
FAU - Eastmond, D A
AU  - Eastmond DA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Autoantibodies)
RN  - 0 (DNA Probes)
RN  - 0 (Mutagens)
RN  - 3L2W5VTT2A (Methylenebis(chloroaniline))
RN  - 56-57-5 (4-Nitroquinoline-1-oxide)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - 4-Nitroquinoline-1-oxide/toxicity
MH  - *Aneuploidy
MH  - Autoantibodies
MH  - B-Lymphocytes
MH  - CREST Syndrome/immunology
MH  - Cell Line
MH  - *Centromere
MH  - *Chromosome Aberrations
MH  - Cyclophosphamide/toxicity
MH  - DNA Probes
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Methylenebis(chloroaniline)/toxicity
MH  - Micronucleus Tests/*methods
MH  - Mutagens/toxicity
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0165-1218(97)00047-5 [pii]
AID - 10.1016/s0165-1218(97)00047-5 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Aug 1;392(1-2):81-95. doi: 10.1016/s0165-1218(97)00047-5.