PMID- 9269756
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 90
IP  - 4
DP  - 1997 Aug 15
TI  - Improved sensitivity of BCR-ABL detection: a triple-probe three-color 
      fluorescence in situ hybridization system.
PG  - 1395-402
AB  - Chronic myeloid leukemia is a clonal stem cell disorder associated with the 
      Philadelphia (Ph) translocation [t(9;22) (q34;q11)]. As a result of the Ph 
      translocation, parts of the ABL and BCR genes become fused. Cytogenetic 
      quantification of Ph+ metaphases can be used to monitor patient response to 
      treatment but is of limited sensitivity and applies only to cycling cells. 
      Fluorescence in situ hybridization (FISH) with probes from the BCR and ABL 
      regions can also identify the Ph translocation in interphase cells. Established 
      systems for the detection of fusion genes by FISH rely on colocalization of two 
      different probes but are associated with a high rate of false-positive results. 
      We have introduced a third probe labeled with a different fluorochrome to create 
      a triple-probe/three-color system that permits identification of both the Ph 
      chromosome and the derivative 9 chromosome in Ph+ cells. This system was used to 
      determine the frequency of interphase cells carrying the BCR-ABL fusion gene in 
      bone marrow and peripheral blood granulocytes from patients showing variable 
      cytogenetic responses to interferon. Our data show that the 
      triple-probe/three-color approach allows highly sensitive detection of residual 
      disease. Moreover, this method is readily applicable to the analysis of other 
      chromosome translocations.
FAU - Sinclair, P B
AU  - Sinclair PB
AD  - Department of Haematology, Addenbrooke's Hospital, Cambridge, UK.
FAU - Green, A R
AU  - Green AR
FAU - Grace, C
AU  - Grace C
FAU - Nacheva, E P
AU  - Nacheva EP
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow Cells
MH  - Chromosome Banding
MH  - DNA Probes
MH  - False Positive Reactions
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Granulocytes/cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/*genetics
MH  - Lymphoma/genetics
MH  - Male
MH  - Middle Aged
MH  - Neoplasm, Residual/diagnosis
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic/genetics
EDAT- 1997/08/15 00:00
MHDA- 1997/08/15 00:01
CRDT- 1997/08/15 00:00
PHST- 1997/08/15 00:00 [pubmed]
PHST- 1997/08/15 00:01 [medline]
PHST- 1997/08/15 00:00 [entrez]
AID - S0006-4971(20)58054-3 [pii]
PST - ppublish
SO  - Blood. 1997 Aug 15;90(4):1395-402.