PMID- 9271437
OWN - NLM
STAT- MEDLINE
DCOM- 19970922
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 17
IP  - 9
DP  - 1997 Sep
TI  - Synergy between suppressor of Hairless and Notch in regulation of Enhancer of 
      split m gamma and m delta expression.
PG  - 5620-8
AB  - The Notch signaling pathway is known to regulate cell fate decisions in a variety 
      of organisms from worms to humans. Although several components of the pathway 
      have been characterized, the actual mechanism and molecular results of signaling 
      remain elusive. We have examined the role of the Notch signaling pathway in the 
      transcriptional regulation of two Drosophila Enhancer of split [E(spl)] genes, 
      whose gene products have been shown to be downstream players in the pathway. 
      Using a reporter assay system in Drosophila tissue culture cells, we have 
      observed a significant induction of E(spl) m gamma and m delta expression after 
      cotransfection with activated Notch. Characterization of the 5' regulatory 
      regions of these two genes led to the identification of a number of target sites 
      for the Suppressor of Hairless [Su(H)] protein, a transcription factor activated 
      by Notch signaling. We show that Notch-inducible expression of E(spl) m gamma and 
      m delta both in cultured cells and in vivo is dependent on functional Su(H). 
      Although overexpression of Su(H) augments the level of induction of the reporter 
      genes by activated Notch, Su(H) alone is insufficient to produce high levels of 
      transcriptional activation. Despite the synergy observed between activated Notch 
      and Su(H), the former affects neither the nuclear localization nor the DNA 
      binding activity of the latter.
FAU - Eastman, D S
AU  - Eastman DS
AD  - Institute of Molecular Biology and Biotechnology, University of Crete, Heraklion, 
      Greece.
FAU - Slee, R
AU  - Slee R
FAU - Skoufos, E
AU  - Skoufos E
FAU - Bangalore, L
AU  - Bangalore L
FAU - Bray, S
AU  - Bray S
FAU - Delidakis, C
AU  - Delidakis C
LA  - eng
SI  - GENBANK/AJ000495
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (E(spl)mdelta-HLH protein, Drosophila)
RN  - 0 (Insect Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (N protein, Drosophila)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Notch)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 147445-80-5 (E(spl)mgamma-HLH protein, Drosophila)
RN  - 148349-66-0 (H protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Binding Sites/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Drosophila
MH  - *Drosophila Proteins
MH  - *Helix-Loop-Helix Motifs
MH  - Insect Proteins/*antagonists & inhibitors
MH  - Membrane Proteins/*antagonists & inhibitors
MH  - Molecular Sequence Data
MH  - Receptors, Cell Surface/*antagonists & inhibitors
MH  - Receptors, Notch
MH  - Repressor Proteins
MH  - *Transcription Factors
PMC - PMC232410
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
PMCR- 1997/09/01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
PHST- 1997/09/01 00:00 [pmc-release]
AID - 10.1128/MCB.17.9.5620 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1997 Sep;17(9):5620-8. doi: 10.1128/MCB.17.9.5620.