PMID- 9272163
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20220321
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 100
IP  - 3-4
DP  - 1997 Sep
TI  - Detection of chromosomal aneuploidy in endometriosis by multi-color fluorescence 
      in situ hybridization (FISH).
PG  - 401-6
AB  - Endometriosis affects 10-15% of women of reproductive age and is a common cause 
      of infertility and pelvic pain. Although endometriosis is characterized by 
      abnormal growth or turn-over of cells, the genetic changes involved remain 
      unclear. We employed a multi-color fluorescence in situ hybridization (FISH) 
      strategy to determine the incidence of somatic chromosomal numeric alterations in 
      severe/late stage endometriosis. Using alpha-satellite sequence-specific DNA 
      probes for chromosomes 7, 8, 11, 12, 16, 17, and 18, simultaneous two- and 
      three-color FISH were performed to evaluate the frequency of monosomic, disomic, 
      and trisomic cells in normal control and endometriotic tissue specimens. In one 
      of four endometriosis samples studied, a significantly higher frequency of 
      monosomy for chromosome 17 (14.8%, chi 2(4) = 53.3, P < 0.0001) and 16 (8.8%, chi 
      2(4) = 11.4, P < 0.05) was observed. An increased number of cells with chromosome 
      11 trisomy (14.8%, chi 2(4) = 96.2, P < 0.0001) were detected in a second case. 
      In a third case, a distinct colony of nuclei with chromosome 16 monosomy (14.1%, 
      chi 2(4) = 21.39, P < 0.005) was detected. Acquired chromosome-specific 
      aneuploidy may be involved in endometriosis, reflecting clonal expansion of 
      chromosomally abnormal cells. That candidate tumor suppressor genes and oncogenes 
      have been mapped to chromosomes 11, 16, and 17 suggests that chromosomal loss or 
      gain plays a role in the development and/or progression of endometriosis.
FAU - Shin, J C
AU  - Shin JC
AD  - Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, 
      Texas 77030, USA.
FAU - Ross, H L
AU  - Ross HL
FAU - Elias, S
AU  - Elias S
FAU - Nguyen, D D
AU  - Nguyen DD
FAU - Mitchell-Leef, D
AU  - Mitchell-Leef D
FAU - Simpson, J L
AU  - Simpson JL
FAU - Bischoff, F Z
AU  - Bischoff FZ
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
SB  - IM
MH  - *Aneuploidy
MH  - Color
MH  - Endometriosis/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - 10.1007/s004390050524 [doi]
PST - ppublish
SO  - Hum Genet. 1997 Sep;100(3-4):401-6. doi: 10.1007/s004390050524.