PMID- 9273358
OWN - NLM
STAT- MEDLINE
DCOM- 19970822
LR  - 20191102
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 127
IP  - 3
DP  - 1996 Mar
TI  - Molecular cloning and expression of a recombinant Aspergillus fumigatus protein 
      Asp f II with significant immunoglobulin E reactivity in allergic 
      bronchopulmonary aspergillosis.
PG  - 253-62
AB  - The cDNA of Aspergillus fumigatus encoding an allergen was cloned and expressed 
      in Escherichia coli. The 987 bp long cDNA clone expressed a recombinant protein 
      Asp f II of 34 kd. This protein exhibited binding to immunoglobulin E (IgE) in 
      the serum samples from patients with allergic bronchopulmonary aspergillosis 
      (ABPA). The patients with ABPA and central bronchiectasis demonstrated high 
      levels of serum IgE antibodies, whereas patients with ABPA without central 
      bronchiectasis, patients with asthma and Aspergillus skin test reactivity but no 
      evidence of ABPA, and patients with aspergilloma showed only low levels of IgE 
      antibody to Asp f II. In two-dimensional electrophoresis, a native antigen 
      electroeluted from an A. fumigatus culture filtrate antigen preparation showed an 
      isoelectric point and molecular weight similar to that of Asp f II. In a 
      competitive enzyme-linked immunosorbent assay (ELISA), the IgE antibody 
      reactivity of Asp f II with patient serum samples could be significantly 
      inhibited by culture filtrate antigens of A. fumigatus. These results indicate 
      that Asp f II has immunologic reactivities comparable to those of native A. 
      fumigatus antigens. The recombinant Asp f II can be expressed in E. coli in large 
      quantities and should prove useful as a standardized allergen for sensitive and 
      specific immunodiagnosis of ABPA, especially in patients with central 
      bronchiectasis.
FAU - Banerjee, B
AU  - Banerjee B
AD  - Department of Medicine, Medical College of Wisconsin, Milwaukee, USA.
FAU - Kurup, V P
AU  - Kurup VP
FAU - Phadnis, S
AU  - Phadnis S
FAU - Greenberger, P A
AU  - Greenberger PA
FAU - Fink, J N
AU  - Fink JN
LA  - eng
SI  - GENBANK/U20722
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antibodies, Fungal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Molecular Probes)
RN  - 0 (Recombinant Proteins)
RN  - 0 (allergen Asp f 2, Aspergillus fumigatus)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/immunology
MH  - Antigen-Antibody Reactions
MH  - Aspergillosis, Allergic Bronchopulmonary/blood/*immunology
MH  - Aspergillus/metabolism
MH  - Base Sequence
MH  - *Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Fungal Proteins/*genetics/*immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Molecular Probes/genetics
MH  - Molecular Sequence Data
MH  - Rabbits
MH  - Recombinant Proteins
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - S0022-2143(96)90093-1 [pii]
AID - 10.1016/s0022-2143(96)90093-1 [doi]
PST - ppublish
SO  - J Lab Clin Med. 1996 Mar;127(3):253-62. doi: 10.1016/s0022-2143(96)90093-1.