PMID- 9275323
OWN - NLM
STAT- MEDLINE
DCOM- 19970923
LR  - 20141120
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 109
IP  - 12
DP  - 1996 Dec
TI  - A study of time window for oxidative stress associated with myocardial ischemia 
      and reperfusion.
PG  - 926-9
AB  - OBJECTIVE: To investigate the time window for oxidative stress occurred in 
      myocardial tissue and associated with reperfusion. METHODS: Thirty nine conscious 
      rabbits were subjected to 0.5 hours (h) of myocardial ischemia and reperfusion 
      for 0 h, 6 h, 24 h and 72 h, respectively, and nine rabbits served as controls. 
      Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and 
      myeloperoxidase (MPO), and contents of GSH and malondyaldehyde (MDA) were 
      measured in myocardial tissues taken from ischemic non-necrotic area (INNA), 
      ischemic necrotic area (INA) and non-ischemic area (NIA), respectively. RESULTS: 
      The antioxidant status of INNA was weakened at 6th h, 24th h, and 48th h of 
      reperfusion. Activities of Mn-SOD and Cu-Zu-SOD returned to normal value at 72nd 
      h of reperfusion, but GSH-px activity and GSH content remained in lower value. 
      MDA content had a marked increase at 6th h, 24th h, 48th/h and 72nd h of 
      reperfusion. MPO activity had a progressive increase after reperfusion, with its 
      peak value at 48th h, and PMN accumulation occurred mostly in necrotic zone and 
      its outer board zone. A mild oxidative stress also occurred in non-ischemic 
      tissue, but without obvious neutrophils infiltration. CONCLUSIONS: The time 
      window for oxidative stress associated with myocardial ischemia and reperfusion 
      lasts for at least 72 h, and PMN infiltration is one of the most important 
      factors of delayed myocardial reperfusion injury.
FAU - Zhao, X
AU  - Zhao X
AD  - Department of Cardiology, Changzheng Hospital, Shanghai.
FAU - Fu, W
AU  - Fu W
FAU - Yuan, W
AU  - Yuan W
FAU - Wu, Z
AU  - Wu Z
FAU - Yang, Z
AU  - Yang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Glutathione Peroxidase/metabolism
MH  - In Vitro Techniques
MH  - Malondialdehyde/metabolism
MH  - Myocardial Ischemia/*metabolism
MH  - Myocardial Reperfusion Injury/*metabolism
MH  - Neutrophils/physiology
MH  - *Oxidative Stress
MH  - Peroxidase/metabolism
MH  - Rabbits
MH  - Superoxide Dismutase/metabolism
MH  - Time Factors
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1996 Dec;109(12):926-9.