PMID- 9275356
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20061115
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 109
IP  - 10
DP  - 1996 Oct
TI  - Alterations of level of total genomic DNA methylation and pattern of c-myc, 
      c-Ha-ras oncogene methylation in human gastric carcinogenesis.
PG  - 787-91
AB  - OBJECTIVE: To investigate the status of DNA methylation in gastric 
      carcinogenesis. METHODS: We analysed methylation pattern of c-myc, c-Ha-ras 
      oncogenes by southern blot hybridization. DNA from cancerous, paracancerous and 
      non-cancerous area of surgically resected samples in 21 cases of advanced gastric 
      cancer were digested with MspI/HpaII and hybridized with the two genomic 32P 
      labelled probes. In addition, the level of total genomic DNA methylation was 
      measured by incubating DNA with 3H-S-Adenosylmethionine (3H-SAM) in the presence 
      of a methylase which methylates all the cytosine residues that are in the double 
      CpG (cytosine-guanine). RESULTS: The results indicated that both c-myc and 
      c-Ha-ras oncogene fragments containing CCGG sequence were hypomethylated in DNA 
      samples from cancerous (10/21 and 5/10) and paracancerous (13/21 and 4/10) areas. 
      Moreover, the level of total genomic DNA methylation in cancerous tissue was 
      significantly lower than that in non-cancerous and paracancerous mucosa tissues 
      (P < 0.05). The results from two methods are incompletely alike. CONCLUSION: 
      These results supported a strong correlation between DNA hypomethylation and 
      gastric carcinogenesis, particularly methylated pattern of c-myc and c-Ha-ras 
      oncogenes fragments containing CCGG sequence was abnormal in gastric mucosa 
      tissue from gastric cancerous and paracancerous areas.
FAU - Fang, J
AU  - Fang J
AD  - Shanghai Second Medical University, Shanghai Institute of Digestive Disease.
FAU - Zhu, S
AU  - Zhu S
FAU - Xiao, S
AU  - Xiao S
FAU - Shi, Y
AU  - Shi Y
FAU - Jiang, S
AU  - Jiang S
FAU - Zhou, X
AU  - Zhou X
FAU - Qian, L
AU  - Qian L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blotting, Southern
MH  - CpG Islands
MH  - *DNA Methylation
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - *Genes, myc
MH  - *Genes, ras
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Stomach Neoplasms/*genetics
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1996 Oct;109(10):787-91.