PMID- 9275656
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 25
IP  - 4
DP  - 1996 Aug
TI  - [Effects of oxidized low density lipoprotein and very low density lipoprotein on 
      the expression of MCP-1 by monocytes].
PG  - 220-3
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a potent chemotatic factor for 
      monocytes. This study aimed at examination of the effects of oxidized low density 
      lipoprotein (OX-LDL) and oxidized very low density lipoprotein (OX-VLDL) on the 
      expression of MCP-1 mRNA and protein by monocytes. The guanidinium isothiocyanate 
      method was used to extract total RNA from monocytes which were exposed to LDL, 
      OX-LDL, VLDL and OX-VLDL for 24 hours respectively and the monocyte derived 
      conditioned media collected. MCP-1 mRNA expression in monocytes was examined by 
      slot blot and Northern blot analysis using a gamma 32P-end-labelled 35 mer 
      oligonucleotide probe of MCP-1. Meanwhile, MCP-1 protein in the conditioned media 
      was examined by use of sandwich ELISA. It was found that monocytes from human 
      peripheral blood can express MCP-1 mRNA and protein after 24 hours incubation at 
      37 degrees C and the expression of MCP-1 mRNA and protein was markedly increased 
      by a 24 hour exposure to OX-LDL and OX-VLDL, whereas the expression of MCP-1 mRNA 
      and protein was only sightly increased when exposed to LDL and VLDL. It is 
      concluded that monocytes can express MCP-1 that induce additional monocyte 
      migration into the subendothelial space, and that OX-LDL and OX-VLDL enhance this 
      function by inducing stronger MCP-1 expression in monocytes.
FAU - Wang, G
AU  - Wang G
AD  - Department of Pathology, Tongji Medical University, Wuhan.
FAU - Deng, Z
AU  - Deng Z
FAU - Qu, Z
AU  - Qu Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (oxidized low density lipoprotein)
RN  - 0 (oxidized very low density lipoprotein)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Humans
MH  - Lipoproteins, LDL/*pharmacology
MH  - Lipoproteins, VLDL/*pharmacology
MH  - Monocytes/*metabolism
MH  - RNA, Messenger/biosynthesis
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 1996 Aug;25(4):220-3.