PMID- 9279150
OWN - NLM
STAT- MEDLINE
DCOM- 19970911
LR  - 20190501
IS  - 1468-2044 (Electronic)
IS  - 0003-9888 (Print)
IS  - 0003-9888 (Linking)
VI  - 77
IP  - 1
DP  - 1997 Jul
TI  - Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by 
      polymerase chain reaction.
PG  - 42-5
AB  - Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in 
      febrile children by qualitative and quantitative polymerase chain reaction (PCR) 
      assays. Diagnosis was based on the differential detection of viral DNA in 
      peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile 
      infants, but none of seven non-febrile controls, were identified with primary 
      infections (three HHV-6, three HHV-7). These children had significantly higher 
      viral loads in PBMC (HHV-6, median 24213 genomes/10(6) PBMC; HHV-7, median 
      6,040,000 genomes/10(6) PBMC) than DNA-aemic, saliva PCR positive children 
      (HHV-6, median 1606 genomes/10(6) PBMC, p < 0.01; HHV-7, median 7089 genomes/ 
      10(6) PBMC, p < 0.05). Viral DNA was detected in serum by PCR in only 50% of 
      primary infections. All three children with primary HHV-7 infection had febrile 
      convulsions. Thus PCR, including quantitative assays, may identify primary HHV-6 
      and HHV-7 infections when an appropriate combination of clinical specimens is 
      used.
FAU - Clark, D A
AU  - Clark DA
AD  - Department of Virology, Royal Free Hospital School of Medicine, London.
FAU - Kidd, I M
AU  - Kidd IM
FAU - Collingham, K E
AU  - Collingham KE
FAU - Tarlow, M
AU  - Tarlow M
FAU - Ayeni, T
AU  - Ayeni T
FAU - Riordan, A
AU  - Riordan A
FAU - Griffiths, P D
AU  - Griffiths PD
FAU - Emery, V C
AU  - Emery VC
FAU - Pillay, D
AU  - Pillay D
LA  - eng
GR  - AI-33389/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Arch Dis Child
JT  - Archives of disease in childhood
JID - 0372434
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA, Viral/*analysis
MH  - Herpesviridae Infections/*diagnosis
MH  - Herpesvirus 6, Human/*genetics
MH  - Herpesvirus 7, Human/*genetics
MH  - Humans
MH  - Infant
MH  - Leukocytes, Mononuclear/virology
MH  - *Polymerase Chain Reaction
MH  - Seizures, Febrile/*virology
MH  - Viral Load
PMC - PMC1717251
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
PMCR- 2000/07/01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
PHST- 2000/07/01 00:00 [pmc-release]
AID - 10.1136/adc.77.1.42 [doi]
PST - ppublish
SO  - Arch Dis Child. 1997 Jul;77(1):42-5. doi: 10.1136/adc.77.1.42.