PMID- 9281465
OWN - NLM
STAT- MEDLINE
DCOM- 19971002
LR  - 20041117
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 13
IP  - 1
DP  - 1997 Sep
TI  - Laboratory analyses in the diagnosis of human allergic disease.
PG  - 25-32
AB  - The clinical immunology laboratory provides support to the allergist in the 
      diagnosis and management of human allergic diseases. Following a clinical 
      history, the detection of allergen-specific immunoglobulin E (IgE) in serum can 
      be useful in the definitive diagnosis of an IgE antibody-mediated 
      hypersensitivity. Total serum IgE, the multiallergen screen, and mast cell 
      tryptase are less commonly measured analytes in the work-up of the allergic 
      patient. More recently, the clinical laboratory has employed monoclonal antibody 
      based-enzyme immunoassays to quantify dust mite, cat, dog, and cockroach 
      indicator aeroallergen levels in indoor home and work environments. Levels of 
      allergenic proteins in environmental and medical/consumer products are measured 
      by other assays such as the competitive inhibition radioallergosorbent test. 
      Methods of quantification, internal quality control, and external proficiency 
      testing programs are emphasized in this report.
FAU - Hamilton, R G
AU  - Hamilton RG
AD  - Johns Hopkins Asthma and Allergy Center, Johns Hopkins University School of 
      Medicine, Baltimore, Maryland 21224, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Glycoproteins)
RN  - 0 (Latex)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/analysis/immunology
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, Dermatophagoides
MH  - Cats
MH  - Consumer Product Safety
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/immunology
MH  - Humans
MH  - Hypersensitivity/*diagnosis/immunology/therapy
MH  - Immunoglobulin E/*blood/immunology
MH  - Latex/immunology
MH  - Quality Control
MH  - Radioallergosorbent Test
MH  - Reference Standards
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - S1046-2023(97)90493-X [pii]
AID - 10.1006/meth.1997.0493 [doi]
PST - ppublish
SO  - Methods. 1997 Sep;13(1):25-32. doi: 10.1006/meth.1997.0493.