PMID- 9281470
OWN - NLM
STAT- MEDLINE
DCOM- 19971002
LR  - 20071114
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 13
IP  - 1
DP  - 1997 Sep
TI  - Immunologic investigations of T-cell regulation of human IgE antibody secretion 
      and allergic responses.
PG  - 69-78
AB  - The pathophysiology of allergic disease is multifactorial, involving an intricate 
      network of interactions among cells, mediators, and cytokines. Substantial 
      progress has been made in defining the role of antigen-specific T cells and 
      cytokines in the regulation of immunoglobulin E (IgE) synthesis and the atopic 
      diseases. The development of antigen-specific T-cell lines and clones has 
      facilitated efforts to characterize human T-cell subsets and their cytokine 
      repertoires. Molecular methods currently available include techniques for the 
      quantitative analysis of cytokine gene expression and secretion from activated T 
      cells ex vivo as well as in tissues. The availability of these newly developed 
      techniques has become essential to the investigation of the pharmacologic 
      regulation of T cells and cytokines both in vitro and in vivo. Future 
      investigations will contribute to our understanding of the differential 
      regulation of T-cell subsets and their relationships to allergic diseases, 
      ultimately leading to a better understanding of the molecular pathogenesis of 
      allergic diseases and the design of more effective therapeutic interventions.
FAU - Essayan, D M
AU  - Essayan DM
AD  - Division of Clinical Immunology, Johns Hopkins University School of Medicine, 
      Baltimore, Maryland 21224, USA.
FAU - Krishnaswamy, G
AU  - Krishnaswamy G
FAU - Huang, S K
AU  - Huang SK
LA  - eng
GR  - AI34002/AI/NIAID NIH HHS/United States
GR  - AI40274/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (Cytokines)
RN  - 0 (Glucocorticoids)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Cytokines/*biosynthesis/genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Glucocorticoids/pharmacology
MH  - Humans
MH  - Hypersensitivity/*immunology/therapy
MH  - Immunoglobulin E/*biosynthesis/immunology
MH  - In Situ Hybridization
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - T-Lymphocyte Subsets/*immunology
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - S1046-2023(97)90498-9 [pii]
AID - 10.1006/meth.1997.0498 [doi]
PST - ppublish
SO  - Methods. 1997 Sep;13(1):69-78. doi: 10.1006/meth.1997.0498.