PMID- 9283589
OWN - NLM
STAT- MEDLINE
DCOM- 19970917
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 97
IP  - 2
DP  - 1997 Sep
TI  - Monoallelic p53 deletion in chronic lymphocytic leukemia detected by interphase 
      cytogenetics.
PG  - 97-100
AB  - Chromosomal aberrations can be detected in 50% of patients with chronic 
      lymphocytic leukemia (CLL). A role for tumor suppressor genes in the genesis of 
      lymphoid tumors has been reported. In B-CLL, p53 gene mutations were found in 
      10-15% of the patients. We used fluorescence in situ hybridization (FISH) to 
      detect p53 deletion in B-CLL. We also correlated the cytogenetic findings with 
      the clinical course. In situ hybridization to interphase nuclei showed monallelic 
      p53 deletion in 6 of 23 patients (26%). The percentage of cells with one p53 
      signal ranged from 12 to 100. A statistically significant correlation between p53 
      deletion and progression of CLL was demonstrated. We conclude that FISH is a 
      sensitive and reliable method to detect deletion of specific genes (i.e., p53) in 
      CLL. The finding of p53 deletion is associated with disease progression.
FAU - Amiel, A
AU  - Amiel A
AD  - Department of Medicine, Meir Hospital, Kfar Saba, Israel.
FAU - Arbov, L
AU  - Arbov L
FAU - Manor, Y
AU  - Manor Y
FAU - Fejgin, M
AU  - Fejgin M
FAU - Elis, A
AU  - Elis A
FAU - Gaber, E
AU  - Gaber E
FAU - Lishner, M
AU  - Lishner M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Alleles
MH  - Cytogenetics/methods
MH  - Female
MH  - *Gene Deletion
MH  - *Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Leukocytes, Mononuclear/ultrastructure
MH  - Male
MH  - Middle Aged
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - S016546089600341X [pii]
AID - 10.1016/s0165-4608(96)00341-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Sep;97(2):97-100. doi: 
      10.1016/s0165-4608(96)00341-x.