PMID- 9283601
OWN - NLM
STAT- MEDLINE
DCOM- 19970917
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 97
IP  - 2
DP  - 1997 Sep
TI  - High frequency of trisomy 8 in acute promyelocytic leukemia: a fluorescence in 
      situ hybridization study.
PG  - 161-4
AB  - Correct diagnosis of acute promyelocytic leukemia (APL) requires proof of the 
      translocation (15;17)(q24;q11), which appears to be absolutely specific for this 
      particular type of myeloid disorder. We studied the karyotypes of 29 consecutive 
      APL patients at diagnosis: in 5 of them banding techniques failed to detect the 
      t(15;17). In these seemingly cytogenetically negative cases, fluorescence in situ 
      hybridization (FISH) with a chromosome 17 painting probe detected a high 
      percentage of mitoses with 3 hybridization signals: one derived from the intact 
      chromosome 17, and 2 from the rearranged chromosomes 15 and 17. Trisomy 8 (+8) as 
      a secondary chromosomal abnormality was observed in 8 cases (27.5%), confirming 
      that the t(15;17) favors the acquisition of an extra chromosome 8. One of these 8 
      cases showed a marker that was interpreted by FISH analysis as der(8) with 
      duplication of a segment of the long arm carrying the c-MYC allele. Clinical 
      features of patients with t(15;17) and +8 were no different from patients with 
      t(15;17) alone. The usefulness of FISH to standard banding techniques in the 
      detection of specific structural and/or numerical chromosomal abnormalities is 
      confirmed in this report.
FAU - Sessarego, M
AU  - Sessarego M
AD  - Department of Internal Medicine, D.I.M.I., University of Genoa, Italy.
FAU - Fugazza, G
AU  - Fugazza G
FAU - Balleari, E
AU  - Balleari E
FAU - Bruzzone, R
AU  - Bruzzone R
FAU - Ballestrero, A
AU  - Ballestrero A
FAU - Patrone, F
AU  - Patrone F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 8
MH  - Genes, myc
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Translocation, Genetic
MH  - *Trisomy
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - S0165460896003238 [pii]
AID - 10.1016/s0165-4608(96)00323-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Sep;97(2):161-4. doi: 10.1016/s0165-4608(96)00323-8.