PMID- 9284919
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 77
IP  - 3-4
DP  - 1997
TI  - Conservation of human vs. feline genome organization revealed by reciprocal 
      chromosome painting.
PG  - 211-7
AB  - We employed fluorescence in situ hybridization (FISH) with probes established by 
      flow sorting metaphase chromosomes of the domestic cat (Felis cattus, 2n = 38) to 
      "paint" homologous segments on human chromosomes and, reciprocally, using human 
      chromosome paints on feline metaphase preparations. The results revealed, by 
      direct microscopic observation, widespread conservation of genome organization 
      between the two mammalian orders and confirmed 90% of the homologous genes mapped 
      to both species. Fourteen of 23 human chomosomes were hybridized with single cat 
      probes, and 9 of 19 cat chromosomes were entirely labeled by a single human 
      probe. All other chromosomes were labeled with only two or, at most, three probes 
      of the respective species. Y-chromosome probes gave no signals. Approximately 30 
      syntenic segments were identified, and the number of translocations could be 
      estimated to be on the order of one new translocation per 10 million years in the 
      phylogenetic lines leading to human and cat. Using the principle of maximum 
      parsimony, the primitive vs. derived human chromosome segments were identified by 
      comparison to the feline, cattle, and pig genomes, a first step in reconstructing 
      the evolutionary heritage of the mammalian radiations. The results suggest that 
      reciprocal chromosome painting will help reconstruct the history of genomic 
      changes by determining the polarity of chromosomal rearrangements and 
      establishing the ancestral karyotype for each principle branching point in 
      mammalian evolution.
FAU - Wienberg, J
AU  - Wienberg J
AD  - Department of Pathology, Cambridge University, UK. jw@mole.bio.cam.ac.uk
FAU - Stanyon, R
AU  - Stanyon R
FAU - Nash, W G
AU  - Nash WG
FAU - O'Brien, P C
AU  - O'Brien PC
FAU - Yang, F
AU  - Yang F
FAU - O'Brien, S J
AU  - O'Brien SJ
FAU - Ferguson-Smith, M A
AU  - Ferguson-Smith MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Molecular Probes)
SB  - IM
MH  - Animals
MH  - Cats/*genetics
MH  - Cattle
MH  - Chromosomes/genetics/ultrastructure
MH  - Chromosomes, Human/genetics/ultrastructure
MH  - Evolution, Molecular
MH  - Flow Cytometry
MH  - *Genome
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Molecular Probes
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Species Specificity
MH  - Swine
MH  - Translocation, Genetic
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000134579 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1997;77(3-4):211-7. doi: 10.1159/000134579.