PMID- 9284923
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20220129
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 77
IP  - 3-4
DP  - 1997
TI  - A panel of radiation hybrids and YAC clones specific for human chromosome 5.
PG  - 232-7
AB  - We report the characterization, by reverse fluorescence in situ hybridization 
      (FISH), of 59 hybrids retaining fragments of human chromosome 5. Most of these 
      hybrids are radiation hybrids generated by gamma irradiating, at low dosage, a 
      monochromosomal hybrid retaining chromosome 5 as its only human contribution. The 
      partial chromosome paints generated from these hybrids will make powerful tools 
      for cytogenetic investigations, especially on the cytogenetic evolution of 
      primates, and examples are reported. The molecular characterization of these 
      hybrids was refined using 74 sequence-tagged sites (STSs), which allowed the 
      physical dissection of chromosome 5 into 71 distinct regions with an average 
      length of 2.7 Mb. The panel, therefore, is also suitable for high-precision 
      subregional mapping of new genes or sequences located on chromosome 5. As an 
      additional resource for cytogenetic studies involving chromosome 5, we report the 
      characterization, by FISH, of 73 YACs from CEPH. The vast majority of these YACs 
      are recognized by at least one of the STSs used for hybrid characterization, thus 
      enabling the integrated use of YACs and partial chromosome paints derived from 
      the hybrids.
FAU - Marzella, R
AU  - Marzella R
AD  - Istituto di Genetica, Facolta di Medicina, Bari, Italy.
FAU - Viggiano, L
AU  - Viggiano L
FAU - Ricco, A S
AU  - Ricco AS
FAU - Tanzariello, A
AU  - Tanzariello A
FAU - Fratello, A
AU  - Fratello A
FAU - Archidiacono, N
AU  - Archidiacono N
FAU - Rocchi, M
AU  - Rocchi M
LA  - eng
GR  - E.0336/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast/*genetics
MH  - Chromosomes, Human, Pair 5/*genetics/ultrastructure
MH  - Chromosomes, Human, Pair 8/genetics/ultrastructure
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Female
MH  - Humans
MH  - *Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Pan troglodytes/genetics
MH  - Sequence Tagged Sites
MH  - Translocation, Genetic
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000134583 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1997;77(3-4):232-7. doi: 10.1159/000134583.