PMID- 9284924
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 77
IP  - 3-4
DP  - 1997
TI  - Comparative analysis of Y chromosome structure in Bos taurus and B. indicus by 
      FISH using region-specific, microdissected, and locus-specific DNA probes.
PG  - 238-41
AB  - Results of fluorescence in situ hybridization (FISH) of Bos taurus and B. indicus 
      Y chromosomes using the bovine locus-specific Y probes BC1.2 and lambda ES6.0 and 
      region-specific probes of B. indicus and B. taurus Y chromosomes, which were 
      generated by microdissection and DOP-PCR, indicate that the Y chromosomes of B. 
      indicus (BIN Y) and B. taurus (BTA Y) differ by a pericentric inversion. Parts of 
      the short and long arms of the Y chromosome in B. taurus and the distal half of 
      the Y chromosome in B. indicus were microdissected, amplified by DOP-PCR, 
      biotinylated, and rehybridized in situ to the corresponding metaphase chromosomes 
      to test the chromosome fragment specificity of the DNA probes. The 
      region-specific painting probes were used for hybridization to metaphase 
      chromosomes of the other species. The DNA painting probes BTA Yp12 and BTA 
      Yq12.1-ter derived from BTA Y hybridized to the distal and proximal halves of BIN 
      Y, respectively. Complex hybridization signals on BTA Yq12.1-->qter were 
      generated with the DNA probe BIN Yqcen-centr (centromere-central) after FISH. The 
      results demonstrate that BTA Yp is homologous to the distal half of BIN Y and 
      that BTA Yq corresponds to the proximal part of BIN Yq. Hybridization of the Y 
      chromosome-specific DNA probes lambda ES6.0 to BTA Yp12-->p11 and near to the 
      telomere of BIN Y and BC1.2 to BTA Yq12-->q13 and to the telomere of BIN Y 
      indicate an opposite orientation of the homologous chromosome fragments BTA Yp 
      and of the distal half of BIN Yq.
FAU - Goldammer, T
AU  - Goldammer T
AD  - Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere, Dummerstorf, 
      Germany. goldamme@fbn.uni-rostock.de
FAU - Brunner, R M
AU  - Brunner RM
FAU - Schwerin, M
AU  - Schwerin M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle/*genetics
MH  - DNA Primers/genetics
MH  - *DNA Probes
MH  - Gene Rearrangement
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Species Specificity
MH  - Y Chromosome/*genetics/*ultrastructure
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000134584 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1997;77(3-4):238-41. doi: 10.1159/000134584.