PMID- 9284928
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 77
IP  - 3-4
DP  - 1997
TI  - Comparative genomic hybridization analysis of Y79 and FISH mapping indicate the 
      amplified human mitochondrial ATP synthase alpha-subunit gene (ATP5A) maps to 
      chromosome 18q12-->q21.
PG  - 253-6
AB  - The four mitochondrial ATP synthase alpha-subunit (ATP5A) genes map to 
      chromosomes 2, 9, 16, and 18. In this study we have refined the localization of 
      two of these genes by fluorescence in situ hybridization (FISH) to metaphase 
      spreads, and further characterised the involvement of ATP5A in the amplification 
      process in the retinoblastoma cell line Y79. Comparative genomic hybridization 
      (CGH) analysis of Y79 indicated that gene amplification was present on both the 
      short arm of chromosome 2 and the long arm of chromosome 18. FISH indicated that 
      the functional ATP5A gene mapped to 18q12-->q21, the same band location 
      identified by CGH analysis of Y79. An ATP5A pseudogene (ATP5AP1) maps to 9p12. 
      Gains in chromosomal material at 18q12-->q21 likely involve hybridization to 
      amplified copies of the ATP5A gene while gains at 2p24 represent hybridization to 
      the MYCN and DDX1 genes, also amplified in Y79.
FAU - Godbout, R
AU  - Godbout R
AD  - Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, 
      Canada.
FAU - Pandita, A
AU  - Pandita A
FAU - Beatty, B
AU  - Beatty B
FAU - Bie, W
AU  - Bie W
FAU - Squire, J A
AU  - Squire JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 18/*genetics/ultrastructure
MH  - Chromosomes, Human, Pair 9/genetics/ultrastructure
MH  - Gene Amplification
MH  - Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mitochondria/*enzymology/*genetics
MH  - Protein Conformation
MH  - Proton-Translocating ATPases/chemistry/*genetics
MH  - Pseudogenes
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000134588 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1997;77(3-4):253-6. doi: 10.1159/000134588.