PMID- 9284956
OWN - NLM
STAT- MEDLINE
DCOM- 19970916
LR  - 20181201
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 172
IP  - 3
DP  - 1997 Sep
TI  - Differential regulation of keratinocyte growth factor and hepatocyte growth 
      factor/scatter factor by different cytokines in human corneal and limbal 
      fibroblasts.
PG  - 361-72
AB  - Corneal epithelial stem cells and transient amplifying cells are located in the 
      limbal and corneal regions, respectively. In a serum-free medium with or without 
      different cytokines, limbal fibroblasts consistently produced greater levels of 
      keratinocyte growth factor (KGF) transcript and protein than corneal fibroblasts, 
      whereas corneal fibroblasts produced greater levels of hepatocyte growth factor/ 
      scatter factor (HGF/SF) transcript and protein than limbal fibroblasts. 
      Expression of HGF/SF transcript and protein was up-regulated mildly by epidermal 
      growth factor (EGF), transforming growth factor-alpha (TGF-alpha), or 
      platelet-derived growth factor B (PDGF-BB) but markedly by interleukin-1 beta 
      (IL-1 beta) and was more pronounced in limbal than in corneal fibroblasts. 
      Expression of KGF transcript was down-regulated by EGF, TGF-alpha, and PDGF-BB, 
      was markedly up-regulated by IL-1 beta, and was more pronounced in limbal than in 
      corneal fibroblasts. Expression of KGF protein was up-regulated markedly by IL-1 
      beta and moderately by PDGF-BB, especially in limbal fibroblasts. TGF-beta 1 
      uniquely turned off transcript and protein expression of HGF/SF and KGF in 
      corneal fibroblasts. Although its transcript levels were similarly down-regulated 
      in limbal fibroblasts, KGF protein levels were paradoxically up-regulated by 
      TGF-beta 1 when added alone or with TGF-alpha or IL-1 beta. These data indicate 
      that KGF and HGF/SF, two fibroblast-derived epithelial mitogens, are expressed 
      differentially by limbal and corneal fibroblasts and are modulated by cytokines 
      activated during epithelial-mesenchymal interactions, suggesting that they may 
      play a different role in modulating corneal epithelial stem cells and transient 
      amplifying cells.
FAU - Li, D Q
AU  - Li DQ
AD  - Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida 33101, 
      USA.
FAU - Tseng, S C
AU  - Tseng SC
LA  - eng
GR  - EY-06810/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cytokines)
RN  - 0 (FGF7 protein, human)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (LIF protein, human)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Lymphokines)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Transforming Growth Factor beta)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 1B56C968OA (Becaplermin)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Becaplermin
MH  - Cells, Cultured
MH  - Cornea/cytology/*metabolism
MH  - Cytokines/*pharmacology
MH  - Epidermal Growth Factor/pharmacology
MH  - Fibroblast Growth Factor 10
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Fibroblast Growth Factor 7
MH  - *Fibroblast Growth Factors
MH  - Fibroblasts/metabolism
MH  - *Gene Expression Regulation
MH  - Growth Inhibitors/pharmacology
MH  - Growth Substances/biosynthesis/*genetics
MH  - Hepatocyte Growth Factor/biosynthesis/*genetics
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - *Interleukin-6
MH  - Leukemia Inhibitory Factor
MH  - Limbus Corneae/cytology/*metabolism
MH  - Lymphokines/pharmacology
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Proto-Oncogene Proteins c-sis
MH  - Transforming Growth Factor alpha/pharmacology
MH  - Transforming Growth Factor beta/pharmacology
EDAT- 1997/09/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-4652(199709)172:3<361::AID-JCP10>3.0.CO;2-9 [pii]
AID - 10.1002/(SICI)1097-4652(199709)172:3<361::AID-JCP10>3.0.CO;2-9 [doi]
PST - ppublish
SO  - J Cell Physiol. 1997 Sep;172(3):361-72. doi: 
      10.1002/(SICI)1097-4652(199709)172:3<361::AID-JCP10>3.0.CO;2-9.