PMID- 9285520
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20061115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 49
IP  - 4
DP  - 1997 Aug 15
TI  - Tenascin-C expression and axonal sprouting following injury to the spinal dorsal 
      columns in the adult rat.
PG  - 433-50
AB  - We have examined the expression and distribution of the extracellular matrix 
      molecule tenascin-C in and around lesions of the thoracic dorsal columns in adult 
      rats 3 days to 8 weeks after injury, using in situ hybridization, 
      immunofluorescence, electron microscopy and immunoelectron microscopy. Numerous 
      tenascin-C mRNA+ cells were present in and around the lesion at 3 days; fewer 
      were present at 14 days and almost none 30 days after injury. Most tenascin-C 
      mRNA+ cells in the spinal cord around the lesion were GFAP+, but most of those 
      within the lesion were not, suggesting that tenascin-C is produced in the injured 
      spinal cord by a subpopulation of astrocytes and by other cells that invade the 
      lesion; these cells may include meningeal cells, macrophages, and Schwann cells. 
      From 3 to 30 days after injury, heavy tenascin-C immunoreactivity was present at 
      the lesion site (especially transections), and there was lighter immunoreactivity 
      around the lesion and in the degenerating dorsal column. The heaviest 
      immunoreactivity was associated with collagen fibrils in areas of expanded 
      extracellular space and with basal laminae (covering Schwann cells and some 
      astrocytes) but tenascin-C was also found close to the surfaces of some OX-42 + 
      macrophages/microglia, leptomeningeal cells, and capillaries. Neurofilament (NF)+ 
      axons grew into the highly tenascin-C-immunoreactive lesion sites, indicating 
      that tenascin-C does not prevent axonal growth into these areas. However, such 
      axons were not coated with tenascin-C except where directly exposed to the 
      extracellular space.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Department of Anatomy and Developmental Biology, University College London, 
      United Kingdom. y.zhang@ucl.ac.uk
FAU - Winterbottom, J K
AU  - Winterbottom JK
FAU - Schachner, M
AU  - Schachner M
FAU - Lieberman, A R
AU  - Lieberman AR
FAU - Anderson, P N
AU  - Anderson PN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (RNA, Messenger)
RN  - 0 (Tenascin)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Axons/chemistry/*physiology/ultrastructure
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Expression/physiology
MH  - In Situ Hybridization
MH  - Microscopy, Immunoelectron
MH  - Nerve Regeneration/physiology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/*metabolism
MH  - Tenascin/*genetics/metabolism
EDAT- 1997/08/15 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/08/15 00:00
PHST- 1997/08/15 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/08/15 00:00 [entrez]
AID - 10.1002/(SICI)1097-4547(19970815)49:4<433::AID-JNR5>3.0.CO;2-9 [pii]
PST - ppublish
SO  - J Neurosci Res. 1997 Aug 15;49(4):433-50.