PMID- 9285592
OWN - NLM
STAT- MEDLINE
DCOM- 19970922
LR  - 20220321
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 388
IP  - 6644
DP  - 1997 Aug 21
TI  - Developmental regulation of MHC class II transport in mouse dendritic cells.
PG  - 787-92
AB  - Dendritic cells (DCs) have the unique capacity to initiate primary and secondary 
      immune responses. They acquire antigens in peripheral tissues and migrate to 
      lymphoid organs where they present processed peptides to T cells. DCs must 
      therefore exist in distinct functional states, an idea that is supported by 
      observations that they downregulate endocytosis and upregulate surface molecules 
      of the class II major histocompatibility complex (MHC) upon maturation. Here we 
      investigate the features of DC maturation by reconstituting the terminal 
      differentiation of mouse DCs in vitro and in situ. We find that early DCs, 
      corresponding to those found in peripheral tissues, exhibit a phenotype in which 
      most class II molecules are intracellular and localized to lysosomes. Upon 
      maturation, these cells give rise to a new intermediate phenotype in which 
      intracellular class II molecules are found in peripheral non-lysosomal vesicles, 
      similar to the specialized CIIV population seen in B cells. The intermediate 
      cells then differentiate into late DCs which express almost all of their class II 
      molecules on the plasma membrane. These variations in class II 
      compartmentalization are accompanied by dramatic alterations in the intracellular 
      transport of the new class II molecules and in antigen presentation. We found 
      that although early DCs could not present antigen immediately after uptake, 
      efficient presentation of the previously internalized antigen occurred after 
      maturation, 24-48 hours later. By regulating class II transport and 
      compartmentalization, DCs are able to delay antigen display, a property crucial 
      to their role in immune surveillance.
FAU - Pierre, P
AU  - Pierre P
AD  - Department of Cell Biology, Yale University School of Medicine, New Haven, 
      Connecticut 06520, USA.
FAU - Turley, S J
AU  - Turley SJ
FAU - Gatti, E
AU  - Gatti E
FAU - Hull, M
AU  - Hull M
FAU - Meltzer, J
AU  - Meltzer J
FAU - Mirza, A
AU  - Mirza A
FAU - Inaba, K
AU  - Inaba K
FAU - Steinman, R M
AU  - Steinman RM
FAU - Mellman, I
AU  - Mellman I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
CIN - Nature. 1997 Aug 21;388(6644):724-5. PMID: 9285579
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation
MH  - B-Lymphocytes/immunology
MH  - Biological Transport
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Islets of Langerhans/cytology
MH  - Lysosomes/metabolism
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phenotype
EDAT- 1997/08/21 00:00
MHDA- 2001/03/23 10:01
CRDT- 1997/08/21 00:00
PHST- 1997/08/21 00:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1997/08/21 00:00 [entrez]
AID - 10.1038/42039 [doi]
PST - ppublish
SO  - Nature. 1997 Aug 21;388(6644):787-92. doi: 10.1038/42039.