PMID- 9285630
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20190706
IS  - 0009-7330 (Print)
IS  - 0009-7330 (Linking)
VI  - 81
IP  - 3
DP  - 1997 Sep
TI  - Endothelial cells of the human microvasculature express epidermal fatty 
      acid-binding protein.
PG  - 297-303
AB  - Epidermal fatty acid-binding protein (E-FABP), previously characterized in human 
      keratinocytes, is a cytoplasmic protein of 15 kD that specifically binds fatty 
      acids (FAs). Previous PAGE-immunoblotting studies indicated that several human 
      tissues display an immunoreactive band with an electrophoretic mobility identical 
      to that of E-FABP. The aim of this study was to determine in which cells, other 
      than keratinocytes, E-FABP might be expressed. By immunohistochemistry, we show 
      that E-FABP is expressed in endothelial cells of the microvasculature of the 
      placenta, heart, skeletal muscle, small intestine, lung, and renal medulla. 
      Interestingly, in lung, a tissue of endodermal origin, E-FABP staining was also 
      localized to secretory cells, ie, Clara cells, goblet cells, and probably a 
      subpopulation of pneumocytes. RNA isolated from cultured human umbilical vein and 
      normal human dermal microvascular endothelial cells was analyzed by 
      reverse-transcriptase polymerase chain reaction (RT-PCR). Southern blotting and 
      sequencing of the cloned RT-PCR products demonstrate that endothelial E-FABP is 
      identical to keratinocyte E-FABP. These data suggest that E-FABP-mediated FA 
      transport occurs at the level of the microvasculature in several FA target 
      organs.
FAU - Masouye, I
AU  - Masouye I
AD  - Department of Dermatology, University Hospital, Geneva, Switzerland.
FAU - Hagens, G
AU  - Hagens G
FAU - Van Kuppevelt, T H
AU  - Van Kuppevelt TH
FAU - Madsen, P
AU  - Madsen P
FAU - Saurat, J H
AU  - Saurat JH
FAU - Veerkamp, J H
AU  - Veerkamp JH
FAU - Pepper, M S
AU  - Pepper MS
FAU - Siegenthaler, G
AU  - Siegenthaler G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (FABP5 protein, human)
RN  - 0 (FABP7 protein, human)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Myelin P2 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Base Sequence
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Epidermis/metabolism
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Fatty Acids/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Microcirculation/cytology/metabolism
MH  - Myelin P2 Protein/genetics/*metabolism
MH  - *Neoplasm Proteins
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Tissue Distribution
MH  - *Tumor Suppressor Proteins
EDAT- 1997/09/01 00:00
MHDA- 1997/09/01 00:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 1997/09/01 00:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - 10.1161/01.res.81.3.297 [doi]
PST - ppublish
SO  - Circ Res. 1997 Sep;81(3):297-303. doi: 10.1161/01.res.81.3.297.