PMID- 9286248
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20190721
IS  - 0163-2116 (Print)
IS  - 0163-2116 (Linking)
VI  - 42
IP  - 8
DP  - 1997 Aug
TI  - Tissue-specific cytokine production during experimental acute pancreatitis. A 
      probable mechanism for distant organ dysfunction.
PG  - 1783-8
AB  - Our purpose was to determine if cytokines are produced systemically during acute 
      pancreatitis. Proinflammatory cytokines are elevated during acute pancreatitis 
      and have been implicated in the progression of pancreatitis-associated multiple 
      organ dysfunction. Whether these mediators are produced within all tissues or 
      very few specific organs is not known. Edematous pancreatitis was induced in 
      adult male mice by IP injection of cerulein. Necrotizing pancreatitis was induced 
      in young female mice by feeding a choline-deficient, ethionine supplemented diet. 
      Animals were sacrificed as pancreatitis worsened, with multiple organs prepared 
      for tissue mRNA and protein analysis by RT-PCR and immunoblotting. Pancreatitis 
      severity was established by histologic grading and serum amylase and lipase. 
      There was no cytokine mRNA or protein detectable prior to the induction of 
      pancreatitis. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta 
      (IL-1 beta) mRNA and protein were detected within the pancreas early in the 
      course of pancreatitis in both models, coinciding with the development of 
      hyperamylasemia (both P < 0.001). Interleukin-6 was produced in the pancreas 
      after pancreatitis was more fully developed (P < 0.001). IL-1 beta and TNF-alpha 
      were subsequently produced in large amounts in lung, liver, and spleen but never 
      within kidney, cardiac muscle, or skeletal muscle. A significant delay between 
      pancreatic and distant organ cytokine production was always observed. It is 
      concluded that proinflammatory cytokines are produced within the pancreas and 
      within organs known to develop dysfunction during severe pancreatitis. Cytokine 
      production is tissue specific, correlates with disease severity, and occurs 
      within the pancreas first and subsequently within distant organs.
FAU - Norman, J G
AU  - Norman JG
AD  - Department of Surgery, University of South Florida, Tampa 33612, USA.
FAU - Fink, G W
AU  - Fink GW
FAU - Denham, W
AU  - Denham W
FAU - Yang, J
AU  - Yang J
FAU - Carter, G
AU  - Carter G
FAU - Sexton, C
AU  - Sexton C
FAU - Falkner, J
AU  - Falkner J
FAU - Gower, W R
AU  - Gower WR
FAU - Franz, M G
AU  - Franz MG
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Actins)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Actins/biosynthesis
MH  - Acute Disease
MH  - Animals
MH  - Cytokines/*biosynthesis
MH  - Female
MH  - Interleukin-1/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Liver/metabolism
MH  - Lung/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Pancreas/metabolism
MH  - Pancreatitis/*metabolism/physiopathology
MH  - Pancreatitis, Acute Necrotizing/metabolism/physiopathology
MH  - Spleen/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1023/a:1018886120711 [doi]
PST - ppublish
SO  - Dig Dis Sci. 1997 Aug;42(8):1783-8. doi: 10.1023/a:1018886120711.