PMID- 9286277
OWN - NLM
STAT- MEDLINE
DCOM- 19970915
LR  - 20151119
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 38
IP  - 9
DP  - 1997 Aug
TI  - Human leukocyte antigen class I expression. Marker of poor prognosis in uveal 
      melanoma.
PG  - 1865-72
AB  - PURPOSE: Because the expression of human leukocyte antigen (HLA) antigens is 
      important for immunologic recognition of tumor cells, we determined expression of 
      locus-specific HLA class I antigens in uveal melanoma and tested whether the 
      level of HLA expression was related to prognosis or associated with known 
      prognostic parameters. METHODS: Expression of HLA-A and -B antigens was 
      determined on 30 formalin-fixed and paraffin-embedded sections of uveal melanoma 
      by immunohistochemistry with locus-specific monoclonal antibodies and scored 
      semiquantitatively. RESULTS: The level of expression of HLA-A and -B varied 
      between uveal melanomas. Expression levels of HLA-A and -B were significantly 
      correlated (P = 0.02). High HLA-B expression was significantly correlated with 
      the presence of epithelioid cells (P = 0.04) in the tumor. Expression levels of 
      HLA-A as well as of HLA-B, cell type, mitotic rate, Mib-1 score, and largest 
      tumor diameter were significant predictive factors for survival. High expression 
      of HLA-A and -B was associated with a decreased survival. Multiple Cox regression 
      analysis with stepwise selection of covariates showed that the contribution of 
      HLA-A expression to survival (P = 0.0003) exceeded that of tumor diameter (P = 
      0.02) and Mib-1 score (P = 0.04). CONCLUSIONS: Lack of expression of HLA-A as 
      well as of HLA-B antigens on uveal melanoma is correlated with a better patient 
      survival. Our data suggest that shedding of uveal melanoma micrometastases with a 
      low expression of HLA class I into the systemic circulation may facilitate their 
      removal and prevent the development of metastases. These findings support a 
      protective role for natural killer cells in the development of metastatic disease 
      in uveal melanoma.
FAU - Blom, D J
AU  - Blom DJ
AD  - Department of Ophthalmology, Leiden University Hospital, The Netherlands.
FAU - Luyten, G P
AU  - Luyten GP
FAU - Mooy, C
AU  - Mooy C
FAU - Kerkvliet, S
AU  - Kerkvliet S
FAU - Zwinderman, A H
AU  - Zwinderman AH
FAU - Jager, M J
AU  - Jager MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*metabolism
MH  - Female
MH  - HLA-A Antigens/*metabolism
MH  - HLA-B Antigens/*metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Melanoma/*metabolism/mortality/pathology
MH  - Middle Aged
MH  - Prognosis
MH  - Retrospective Studies
MH  - Survival Rate
MH  - Uveal Neoplasms/*metabolism/mortality/pathology
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1997 Aug;38(9):1865-72.