PMID- 9286356
OWN - NLM
STAT- MEDLINE
DCOM- 19971114
LR  - 20211203
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 417
DP  - 1997
TI  - Mannose receptor mediated uptake of antigens strongly enhances HLA-class II 
      restricted antigen presentation by cultured dendritic cells.
PG  - 171-4
AB  - Dendritic cells (DCs) use macropinocytosis and mannose receptor mediated 
      endocytosis for the uptake of exogenous antigens. Here we show that the 
      endocytosis of the mannose receptor and mannosylated antigen is distinct from 
      that of a non-mannosylated antigen. Shortly after internalization, however, both 
      mannosylated and non-mannosylated antigen are found in an MIIC like compartment. 
      The mannose receptor itself does not reach this compartment, and probably 
      releases its ligand in an earlier endosomal structure. Finally, we found that 
      mannosylation of peptides strongly enhanced their potency to stimulate HLA class 
      II-restricted peptide-specific T cell clones. Our results indicate that 
      mannosylation of antigen leads to selective targeting and subsequent superior 
      presentation by DCs which may be useful for vaccine design.
FAU - Tan, M C
AU  - Tan MC
AD  - Department of Immunohematology, Leiden University Hospital, The Netherlands.
FAU - Mommaas, A M
AU  - Mommaas AM
FAU - Drijfhout, J W
AU  - Drijfhout JW
FAU - Jordens, R
AU  - Jordens R
FAU - Onderwater, J J
AU  - Onderwater JJ
FAU - Verwoerd, D
AU  - Verwoerd D
FAU - Mulder, A A
AU  - Mulder AA
FAU - van der Heiden, A N
AU  - van der Heiden AN
FAU - Ottenhoff, T H
AU  - Ottenhoff TH
FAU - Cella, M
AU  - Cella M
FAU - Tulp, A
AU  - Tulp A
FAU - Neefjes, J J
AU  - Neefjes JJ
FAU - Koning, F
AU  - Koning F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Ligands)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Antigen Presentation
MH  - Antigens/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism
MH  - Endocytosis
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Humans
MH  - *Lectins, C-Type
MH  - Ligands
MH  - Mannose Receptor
MH  - *Mannose-Binding Lectins
MH  - Proteins/immunology/metabolism
MH  - Receptors, Cell Surface/*immunology
MH  - Subcellular Fractions/immunology/metabolism
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/978-1-4757-9966-8_28 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 1997;417:171-4. doi: 10.1007/978-1-4757-9966-8_28.